Am.  Jour.  Pharm. 
September,  18i)9.  , 
British  PJiarmaceutical  Conference. 
443 
This  is  run  into  a  separator,  the  dish  carefully  rinsed  with  10  drops  of  water, 
and  then  with  15  c.c.  of  chloroform,  the  whole  being  transferred  to  the  sepa- 
rator. An  excess  of  ammonia  is  added,  and  the  mixture  well  shaken,  and  al- 
lowed to  stand  until  the  chloroform  has  separated.  This  is  run  off,  and  the 
agitation  and  separation  repeated  with  two  successive  quantities  of  5  c.c.  of 
chloroform.  The  chloro formic  solutions  are  bulked,  and  the  alkaloids  ex- 
tracted by  shaking  with  three  successive  quantities  of  10  c.c.  1  per  cent,  sul- 
phuric acid.  The  acid  alkaloidal  solutions  are  drawn  off  in  turn  and  mixed. 
The  alkaloids  are  finally  recovered  from  this  solution  by  repeating  the  treat- 
ment with  ammonia  and  chloroform.  The  solution  of  the  alkaloids  in  chloro- 
form is  then  evaporated  in  a  tared  dish  over  a  water-bath  until  all  chloroform 
has  been  removed.    The  weight  is  taken,  and  the  alkaloidal  residue  titrated 
N  N 
with  —  HC1  and  —q  NaHO,  as  previously  described. 
The  modified  process,  for  employment  in  laboratories  when  economy  of  time 
is  an  object,  is  as  follows  : 
Two  c.c.  of  the  fluid  extract  is  acidified  and  evaporated,  and  the  alkaloids 
extracted  with  chloroform,  as  described  under  No.  3.  The  chloroformic  solution 
of  the  alkaloids  is  evaporated  to  dryness  and  the  residue  titrated  at  once. 
The  following  results  were  obtained  : 
No.  1. 
No.  2. 
NO.  3. 
No.  4. 
Weight. 
Titra- 
tion. 
Weight. 
Titra- 
tion. 
Weight. 
Titra- 
tion. 
Weight. 
Titra- 
tion. 
B.P.  process 
1-92 
r6o 
1-64 
1-26 
m 
1-41 
rSS 
1-25 
Wilson's  " 
2-23 
i-93 
1 -85 
1 '57 
i-97 
1*64 
i*95 
1-62 
F.  &  W.  " 
220 
2-02 
1*90 
178 
2-04 
1-82 
206 
1-73 
Quick  " 
'"7 
174 
r8o 
172 
For  the  determination  of  the  alkaloidal  value  of  the  wine  and  vinegar,  the 
following  modification  is  given  : 
Fifty  c.c.  of  the  sample  is  placed  in  a  porcelain  dish,  10  drops  of  diluted  sul- 
phuric acid  added,  and  the  liquid  evaporated  to  about  5  c.c.  It  is  then  trans- 
ferred to  a  separator,  the  dish  rinsed  with  a  few  drops  of  water  and  10  c.c.  of 
chloroform,  and  the  alkaloids  separated  and  determined  exactly  as  described  in 
the  process  recommended  for  the  fluid  extract. 
Several  commercial  samples  of  the  wine  were  determined  by  this  method 
with  the  results  shown  in  the  table  on  following  page. 
The  alkaloids  from  the  wine  were  almost  colorless,  and  the  titration  results 
show  that  they  are  yielded  in  an  almost  perfectly  pure  condition.  This  is  evi- 
dently attributable  to  the  fact  that  the  impurity  present  in  the  crude  alkaloids 
from  the  liquid  extract,  and  which  is  most  probably  of  a  resinous  nature,  is 
thrown  down  and  filtered  out  in  the  process  of  conversion  into  the  wine. 
The  vinegar  was  not  examined,  but  as  it  is  prepared  by  simple  dilution  of  the 
fluid  extract,  it  is  evident  that  the  process  employed  for  the  wine  is  equally 
applicable  to  this  preparation. 
