Am.  Jour.  Pharm. 
April,  1891. 
}     Koch's  Lymph  and  its  Dilutions. 
171 
The  usual  dose  of  the  pure  lymph  is  from  t^<j  cc.  to  ^  cc, 
administered  hypodermically,  but  the  lymph  itself  is  never  used. 
In  its  place  is  employed  one  of  three  solutions — a  10  per  cent.,  a  1 
per  cent,  or  a  iVth  of  1  per  cent,  solution.  The  medium  strength 
is  in  greatest  demand.  The  weakest  strength  is  used  for  children. 
If  a  solution  becomes  turbid  on  standing  it  is  unfit  for  use. 
For  the  purpose  of  administration,  Dr.  Koch  has  devised  a  special 
hypodermic  syringe,  having  a  rubber-bulb  and  a  stop-cock  at  one 
end,  to  which  is  attached  a  glass  tube  ground  at  both  ends  (one  end 
for  the  bulb  attachment;  the  other  for  the  hypodermic  needle), 
having  graduated  upon  it  1  cc.  in  ten  subdivisions.  This  syringe  is 
also  very  convenient  with  which  to  make  the  solutions. 
All  apparatus  used  in  preparing  the  dilutions  should  be  first  thor- 
oughly washed  with  a  solution  of  carbolic  acid  (m),  and  the 
syringe  cleansed  first  with  absolute  alcohol  and  then  with  the  car- 
bolized  solution.    For  making  the  latter  use  boiled,  distilled  water. 
The  formulas  of  the  several  solutions  are  as  follows: 
LYMPH  SOLUTION  NO.  I. 
Lymph,   %  cc. 
Carbolized  water  (^q),  .  .  4>2  cc. 
Wit  h  a  pipette  of  1  cc.  capacity  (graduated  into  to)  measure 
y2  cc.  of  the  lymph,  and  deliver  it  into  a  5  cc.  measure.  Wash 
pipette  twice  with  the  diluting  liquid,  add  the  washings  to  the 
lymph  contained  in  the  graduate  and  dilute  the  whole  to  5  cc. 
Label:  Lymph  Solution  No.  1.  (10 per  cent.) 
Bach  cc.  equals, 
Or  1  cc.  equals,  .  . 
LYMPH  SOLUTION  NO.  2. 
Lymph  Solution  No.  i, 
Carbolized  water  (^o), 
Mix  as  before. 
4%  cc. 
Label :  Lymph  Solution  No.  2.  (/ per  cent.) 
Bach  cc.  equals, 
Or  1  cc.  equals,  .  . 
T1J it  cc- 
LYMPH  SOLUTION  NO.  3. 
Lymph  Solution  No.  2, 
Carbolized  water  (^thy), 
Mix  as  before. 
Yz  cc. 
AY%  cc. 
Label :  Lymph  Solution  No.  3.  (TV  of  1  per  cent.) 
Bach  tl  cc.  equals, 
Or  1  cc.  equals,  . 
