218 
Solatium  Carolmense. 
Am.  Jour.  Pharm 
May,  1891. 
aqueous  solution  of  the  alcoholic  extract  is  acidified  and  agitated 
with  ether.  On  evaporating  the  ether,  there  will  be  found  a  mass 
of  well-defined  feathery  crystals,  soluble  in  alcohol  and  ether.  The 
accompanying  microscopical  drawing  will  illustrate  the  appearance 
under  an  amplification  of  480  diam. 
After  a  number  of  experiments,  I  found  the  following  process  to 
answer  best  for  the  separation  of  the  alkaloid  :  To  the  acidified 
alkaloidal  extract  add  ammonium  hydrate  in  excess  and  agitate  with 
amylic  alcohol,  separate  the  amylic  alcohol  and  agitate  it  with  dilute 
HC1  or  H2S04.  The  acid  solution  is  evaporated  over  H2S04.  The 
alkaloid  as  hydrochloride  or  sulphate  has  the  same  properties  as 
enumerated  in  my  previous  papers. 
Another  experiment  was  made  with  50  gm.  unbruised  fresh 
berries  to  find  out  whether  it  is  necessary  for  the  extraction  of  the 
alkaloid  to  bruise  the  berries  or  not.  After  8  days  maceration  in 
officinal  alcohol,  the  tincture  was  tested  for  alkaloids  in  the  way 
described  above  : 
The  weight  of  Mayer's  precipitate  from  50  gm.  of  bruised 
berries,  0*386  gm. 
50  gm.  unbruised  berries,  °"3°5  gm- 
It  would  seem  justifiable  to  presume  that  bruising  the  fresh  berries 
does  not  effect  an  increased  yield. 
Moisture  present  in  fresh  berries,  77 '53  Ver  cent. 
The  complete  analysis  was  carried  out  with  25  gm.  of  dried 
berries  : 
Petroleum  ether  extract  : 
Volatile  oil,  0*220 
Wax  and  fat,  :  7-I6o 
  7-38o 
Ether  extract : 
Soluble  in  dilute  HC1  (solanidine),  0-574 
Fat  and  resin,  1*214 
  1-788 
Alcohol  extract : 
Solanic  acid  0*300 
Solanine,  0*796 
Resin,  0*592 
Glucose,  0*988 
Extractive,   4 '244 
  6.920 
