336 
Sabbatia  Angularis. 
Am.  Jour.  Pharm. 
July,  1891. 
Solvents  used.  Substances  obtained.  Per  Cent. 
Dilute  soda  solution,  Pectin  and  albuminoids,  ....  6*o6 
Dilute  hydrochloric  acid,  .  .    .  .  Pararabin,  i'20 
Extractive,  2*98 
  4-18 
Chlorine  water,   7-40 
Nitric  acid  and  potassium  chlorate,   13*90 
Cellulose,   36'50 
Ash,  soluble  in  water,  1*17 
Soluble  in  HC1,  1-30 
Insoluble,    -38 
  2-85 
Moisture,   8 '05 
Loss,   -83 
Total,   100  *oo 
With  a  view  of  confirming  the  previous  analysis  and  trying  to 
isolate  the  bitter  principle,  two  hundred  grams  of  the  powdered 
drug  were  percolated  with  five  hundred  cubic  centimeters  of  petrol- 
eum ether,  to  remove  fat,  wax,  etc.  The  drug  was  then  dried  and 
percolated  with  eight  hundred  cubic  centimeters  of  stronger  ether, 
again  dried  and  percolated  with  95  per  cent,  alcohol  until 
exhausted  of  bitter  principle. 
Ethereal  Percolate. — After  recovering  ether  the  residue  was  dis- 
solved in  a  small  quantity  of  absolute  alcohol,  and  poured  into,  water 
containing  one  per  cent,  of  hydrochloric  acid,  the  greenish  resin  so 
precipitated  removed  by  filtration  and  the  filtrate  agitated  with 
chloroform,  which  apparently  exhausted  it.  The  chloroform  was 
separated  and  allowed  to  evaporate  spontaneously,  leaving  a  quan- 
tity of  resinous  matter;  this  was  washed  with  stronger  ether,  which, 
on  spontaneous  evaporation,  left  a  minute  quantity  of  a  substance, 
crystalline  in  character,  reddish-yellow  in  color,  of  a  sharp  acid  taste, 
and  having  a  strong  and  pungent  odor,  resembling  that  of  nicotine. 
It  was  soluble  in  water,  alcohol  and  ether,  and  gave  negative  results 
towards  alkaloidal  reagents.  A  small  quantity  dissolved  in  water 
acidulated  with  hydrochloric  acid  and  placed  in  a  water-bath  at  6o° 
C.  for  one  hour,  and  then  made  alkaline  with  sodium  hydrate  failed 
to  reduce  Fehling's  solution,  indicating  its  non-glucosidal  character. 
It  undoubtedly  was  the  principle  isolated  by  Huneker  in  187 1,  and 
called  by  him  erythrocentaurin. 
Alcoholic  Percolate. — After  recovering  the  solvent  from  this  perco- 
late, a  considerable  amount  of  a  soft  and  very  bitter  extract  was 
