Am.  Jour.  Pharm. 
July,  1891. 
Pancreatic  Juice  in  Man. 
359 
a  O  Oi  per  cent,  solution  of  acetaldehyde  in  alcohol  of  500  is  a  con- 
venient quantity  to  employ  as  a  first  standard. 
Higher  alcohols. — 100  cc.  of  the  distilled  liquid  is  mixed  with  I 
cc.  of  aniline  and  I  cc.  of  phosphoric  acid  of  45 0  B.  to  retain  the 
aldehydes,  and  after  cohobating  for  an  hour,  is  distilled  to  dryness 
in  a  solution  of  salt.  The  distillate  is  treated  with  sulphuric  acid  of 
66°,  and  the  coloration  is  compared  with  that  given  by  an  alcohol 
containing  0-250  gram  of  isobutyl  alcohol  per  litre. 
Nitrogen  compounds. — TOO  cc.  of  the  undistilled  liquid  is  distilled 
to  dryness  with  2  cc.  of  a  solution  of  phosphoric  acid  of  45 0  B.,  the 
residue  is  dissolved  in  about  1,000  cc.  of  distilled  water,  mixed  with 
10  grams  of  sodium  carbonate,  and  distilled,  the  distillate  being 
nesslerized.  Alkaline  potassium  permanganate  is  then  added,  and 
distillation  and  nesslerizing  is  repeated.  The  first  operation  gives 
the  nitrogen  present  as  ammonium  compounds  and  amides,  and  the 
second  gives  the  nitrogen  existing  as  pyridine  bases  and  alkaloids. 
Several  analyses  of  brandy  and  alcohol  are  given. 
CHEMICAL  ANALYSIS   OF  THE  PANCREATIC  JUICE 
IN  MAN.1 
By  J.  Zawadzki. 
The  author  states  that  all  our  knowledge  of  the  pancreatic  juice 
has  been  derived  hitherto  from  experiments  on  the  lower  animals. 
In  man,  as  yet,  only  two  analyses  have  been  made,  and  these  in  the 
case  of  juice  obtained  post-mortem. 
The  pancreatic  juice  analyzed  by  Zawadzki  was  obtained  from  a 
girl,  who  had  bee-n  operated  upon  for  the  removal  of  a  pancreatic 
cyst.  For  14  days  an  abundant  secretion  appeared,  at  first  puru- 
lent, then  watery.    After  four  weeks  the  wound  healed  completely. 
The  pancreatic  juice  for  24  hours  was  collected  and  analyzed.  It 
was  tenacious,  yellowish,  turbid,  and  had  an  alkaline  reaction.  The 
deposit  contained  large  epithelial  cells,  mucous  corpuscles,  and  fine 
granular  detritus,  but  no  crystals.  In  the  ashes  were  found  com- 
pounds of  carbonic,  sulphuric,  phosphoric  and  hydrochloric  acids. 
The  bases  were  sodium,  calcium,  potassium  and  iron.  The  juice 
contained  coagulable  albumen  and  hemi-albumose,  but  no  peptone. 
By  the  action  of  the  juice  on  starch  for  five  hours,  at  a  suitable 
1  Oest.-ungar.  Centralblatt  fur  die  medicinischen  IVissenchaften,  No.  6, 
1891  ;  reprinted  from  The  Medical  Chronicle,  April. 
