AmAu°gU?if9iarm'}  Haplopappus  Baylahuen.  381 
(a)  The  volatile  oil  was  estimated  by  the  method  of  Osse  [Archtv 
d.  Pharm.  [3]  7,  104)  -665  Gm.  was  found. 
(b)  After  the  estimation  of  (a)  the  remainder  of  the  petroleum 
spirit  extract  was  evaporated  to  dryness,  the  temperature  then 
raised  to  no0  C,  water  was  then  added  and  the  residue  again 
evaporated  to  dryness.  The  residue  was  treated  with  a  small 
quantity  of  petroleum  spirit,  when  the  spirit  evaporated.  No  fixed 
oil  could  be  detected.  The  resin,  which  weighed  -479  Gm.,  was  of 
a  green  color,  and  easily  soluble  in  cold  solution  of  potassium 
hydroxide. 
IV.  After  the  drug  had  been  thoroughly  washed  with  petroleum 
spirit,  it  was  dried  at  the  ordinary  temperature  in  a  current  of  dry 
air,  then  macerated  for  seven  days,  with  occasional  shaking,  in  100 
cc.  of  ether,  which  had  been  allowed  to  stand  over  calcium  chloride, 
then  distilled. 
(a)  The  ether  extract  was  evaporated  to  dryness  in  a  current  of 
dry  air.  The  residue  was  treated  with  water,  which  took  up  a  sub- 
stance which  had  a  pleasant  acetic  odor  and  was  acid  to  litmus,  but 
which  was  present  in  such  small  quantity  that  no  attempt  was 
made  at  quantitative  estimation. 
(£)  After  treatment  with  water,  the  residue  was  placed  in  a  desic- 
cator over  sulphuric  acid  until  dry,  when  it  was  treated  with  petro- 
leum spirit,  but,  as  before,  no  fixed  oil  could  be  extracted.  The 
residue  1-329  Gm,  was  a  soft  resin. 
V.  After  dissipation  of  the  ether,  the  drug  v/as  macerated  in 
100  cc.  of  absolute  alcohol,  for  seven  days,  with  occasional  shaking. 
(a)  The  alcoholic  extract  was  partly  evaporated  under  diminished 
pressure,  then  finished  on  the  water  bath.  The  residue  was  treated 
with  water,  which  took  up  -028  Gm.  This  water  extract  had  an 
acid  reaction,  was  turned  slightly  green  by  ferric  chloride,  and  was 
precipitated  by  lead  acetate. 
(b)  The  remainder  of  the  residue,  -307  Gm.  was  a  greenish  yellow 
resin,  soluble  in  potassium  hydroxide  and  in  dilute  ammonia  water. 
VI.  The  drug,  after  extraction  with  alcohol,  was  then  mac- 
erated for  two  days,  with  occasional  shaking,  with  ioocc.  of  water. 
(a)  An  aliquot  part  of  the  water  extract  was  taken,  mixed  with 
two  volumes  of  absolute  alcohol,  allowed  to  stand  in  an  ice  chest  for 
two  days.  The  precipitate  was  then  collected,  washed  with  66  per 
cent,  alcohol,  -146  Gm.  was  found,  which  consisted  of  mucilage, 
together  with  some  albuminoid  matter. 
