382 
Haplopappus  Baylahncn. 
Am.  Jour.  Pharm 
Aug.,  1891. 
(b)  The  filtrate  and  washings  from  (a)  were  evaporated  to  a  small 
bulk,  then  4  volumes  of  absolute  alcohol  were  added.  A  precipi- 
tate was  formed  which  weighed  -262  Gm.  This  precipitate  con- 
sisted of  dextrin  and  allied  substances. 
(c)  The  nitrate  and  washings  from  (b)  were  heated  to  drive  off  the 
alcohol,  then  lead  acetate  was  added  and  the  solution  set  aside  for 
two  days.  The  precipitate  was  collected  and  ignited  according  to 
the  directions  given  by  Fresenius  {Quantitative  analysis,  1881,  299, 
[b]).  The  organic  matter  in  the  precipitate  was  -227  Gm.,  which 
may  be  noted  as  organic  acids,  of  which  tannic  acid  constitutes  the 
largest  part. 
(d)  The  remaining  -347  Gm.  of  the  water  extract  consisted  of 
coloring  matter,  albuminoids,  etc. 
VII.  The  drug  while  still  moist  was  macerated  with  1 00  cc.  of  a 
•2  per  cent,  solution  of  sodium  hydroxide  for  one  day. 
(a)  An  aliquot  part  of  the  solution  was  mixed  with  3  volumes  of 
90  per  cent,  alcohol,  and  allowed  to  stand  24  hours  in  an  ice  chest. 
The  precipitate  weighed  -142  Gm.  It  consisted  of  mucilaginous 
substances  (pectin)  and  albuminoids. 
(b)  The  filtrate  from  (a)  was  evaporated  to  dryness,  weighed,  and 
then  ignited  ;  the  loss  was  -222  Gm.,  which  was  mostly  decompo- 
sition products  and  albuminoids  not  precipitated  by  alcohol. 
VIII.  The  residue  from  VII  was  washed  with  water,  then  macer- 
ated in  100  cc.  of  a  1  per  cent,  solution  of  hydrochloric  acid.  No 
starch  could  be  found  under  the  microscope,  so  only  the  estimation 
of  parabin  and  calcium  oxalate  was  found  necessary. 
(a)  The  solution  was  raised  to  boiling,  filtered  while  hot,  an 
aliquot  part  neutralized  with  ammonia,  then  3  volumes  of  90  per 
cent,  alcohol  were  added.  The  precipitate  was  collected,  dried  and 
weighed,  then  ignited  ;  the  loss,  which  was  -340  Gm.,  was  parabin. 
(b)  The  remaining  calcium  oxide  was  calculated  to  oxalate,  which 
was  -143  Gm. 
IX.  The  residue  from  VIII  was  first  washed  with  water,  then 
alcohol,  and  then  with  ether,  finally  dried,  weighed,  then  ignited, 
the  difference  being  cellulose,  lignin,  etc.,  which  was  3  75 2  Gm. 
Summary : 
Moisture,  I. 
Ash,  II.,  . 
Per  Cent. 
2-II 
12*67 
