390  Cannabis  Indica.  {Am  Augr;is£arm* 
allowed  to  stand  for  one-half  hour,  occasionally  agitated,  the 
ethereal  solution  drawn  off,  the  operation  repeated  with  50  cc. 
of  ether,  and  the  water  solution  rejected.  The  ethereal  solutions 
were  united  and  evaporated  to  dryness  on  a  water  bath.  The 
residue  which  remained  was  in  larger  quantity,  of  the  same  color, 
consistence  and  odor  as  that  found  in  the  previous  method.  The 
quantity  obtained  from  one  kilo  of  the  drug  was  about  75  m.  g.  A 
portion  of  the  residue  made  into  a  chloride  gave  the  same  alka- 
loidal  reactions,  but  more  abundant  precipitates  were  obtained. 
A  small  portion  of  the  residue  gave  the  following  test  for 
nitrogen  :  About  one  m.  g.  of  the  residue  was  placed  in  a  test  tube 
with  2  or  3  m.  g.  of  metallic  sodium,  fused  together  by  means  of  a 
Bunsen  flame  for  2  or  3  minutes,  cooled,  10  cc.  of  water  added, 
filtered,  heated  with  4  or  5  m.  g.  of  FeS04,  acidulated  with  H2S04, 
a  drop  or  two  of  solution  of  Fe2Cl6  added,  at  first  the  solution 
turned  pale  blue  ;  upon  standing  a  short  time  a  blue  precipitate  of 
prussian  blue  settled  at  the  bottom  cf  the  test  tube.  (Conversion 
of  the  nitrogen  into  cyanogen.  Prof.  A.  B.  Prescott's  Organic 
Analysis,  p.  199). 
In  forming  salts,  a  crystalline  sulphate  was  obtained  in  the  follow- 
ing manner:  About  50  m.  g.  of  the  residue  was  neutralized  with 
very  dilute  H2S04,  allowed  to  evaporate  to  dryness,  this  residue 
dissolved  in  alcohol,  the  alcoholic  solution  allowed  to  evaporate 
spontaneously,  crystals  were  formed  which  could  be  seen  with  the 
naked  eye.  Under  the  microscope  they  appeared  to  be  long  needle- 
shaped  crystals,  and  of  a  white  color. 
In  making  a  quantitative  estimation,  a  method  in  which  the  fluid 
extract  is  used,  proposed  by  J.  U.  Lloyd,  was  followed  out,  as  fol- 
lows :  100  cc.  were  rubbed  with  20  cc.  of  solution  of  perchloride  of 
iron  (U.  S.  P.  strength),  the  mixture  triturated  with  NaHC03  until 
a  stiff  magma  was  formed.  This  magma  was  abstracted  with 
chloroform,  using  50  cc.  four  different  times  and  decanted  after 
each  addition.  The  chloroformic  solutions  were  united,  extracted  by 
rotating  in  a  separatory  funnel  with  75  cc.  of  dilute  H2S04  ( 1— 50) 
two  different  times,  and  the  chloroformic  solution  now  rejected. 
The  water  solutions  united,  were  extracted  with  ether,  using  50  cc. 
two  different  times  (this  extracts  the  chlorophyll),  and  the  ethereal 
solution  rejected.  The  water  solution  was  made  alkaline  with 
NaOH,  shaken  out  two  different  times  with  chloroform,  using  50 
