82 
Wine  of  Beef  and  Iron. 
Am.  Jour.  Pharm. 
Feb.,  1890. 
Solids  were  estimated  by  evaporating  a  definite  volume 
(20  cc.)  in  a  platinum  capsule  and  drying-  the  residue  at 
i  io°  C,  until  the  loss  did  not  exceed  a  milligram  per  hour. 
This  residue,  by  incinerating  to  a  constant  weight,  yielded 
the  ash. 
From  a  solution  of  the  latter  in  dilute  hydrochloric  acid, 
the  iron  was  precipitated  as  hydrate  by  ammonia.  After 
thoroughly  washing,  the  precipitate  was  redissolved,  in  hydro- 
chloric acid  and  precipitated  a  second  time  by  an  excess  of 
potassium  hydrate  to  separate  any  alumina  present.  This 
precipitate  collected,  washed,  dried  and  ignited,  yielded  the 
iron  as  Fe203.  The  presence  of  phosphates  did  not  interfere, 
as  was  proven  by  precipitating  the  iron  in  a  parallel  experi- 
ment as  basic  acetate. 
Phosphoric  acid  was  estimated  in  the  ash  by  precipitating 
it  from  a  solution  of  the  latter,  first  as  ammonium  phospho- 
molybdate  and  subsequently  from  the  ammoniacal  solution 
of  the  latter  as  ammonium  magnesium  phosphate.  This 
precipitate  was  collected,  washed,  dried,  ignited,  and  the 
residue  weighed  as  Mg2P207,  calculating  therefrom*  the  phos- 
phoric acid. 
To  determine  the  amount  insoluble  in  alcohol  25  cc.  were 
evaporated  on  a  water-bath  to  5  cc,  and  50  cc.  of  strong  alco- 
hol added  while  stirring.  When  allowed  to  stand  sufficiently 
long,  it  was  found  that  the  precipitate  deposited  on  the  sides 
and  bottom  of  the  beaker,  so  that  the  clear  liquid  could  be 
decanted.  When  not  perfectly  clear,  the  liquid  was  filtered 
and  the  beaker  then  carefully  rinsed  with  a  little  alcohol, 
passing  the  washings  through  the  same  filter.  Any  residue 
on  the  filter  was  then  washed  back  into  the  beaker  with  a 
little  distilled  water,  and  the  entire  precipitate — in  most 
cases  completely  soluble^transf erred  to  a  platinum  dish. 
The  solid  matter  and  finally  the  ash  were  estimated  in  this 
as  above. 
The  total  nitrogen  was  determined  by  Varrentrapp  and 
Will's  method  of  heating  in  a  closed  tube  with  soda  lime, 
absorbing  the  NH3  generated  in  a  measured  quantity  of 
standard  acid,  and  then  titrating  the  latter  with  standard 
alkali.     Every  cc.  of  normal  acid  neutralized  by  NH3  is 
