290  Malt  Extracts  with  Cod  Liver  Oil.  {Amjunae'"i89oarm" 
serve  yet.  The  editor  of  the  Pharm.  Rundschau  filled  last  year 
(Rundschau,  1889,  p.  218)  to  some  extent  another  deficiency  in  this 
branch  of  analytical  work  and  quoted  many  analyses  of  infant  food, 
lactated  food,  milk  food  and  farinaceous  food. 
But  I  cannot  find  reports  on  assays  of  malt  extracts  containing 
cod  liver  oil,  and  offer  a  few  of  mine,  considering  their  place  to  be 
among  food  analyses.  I  will  describe  first  the  simple  method 
followed : 
A—  25  grm.  of  the  preparation  containing  malt  extract  with 
cod  liver  oil  is  boiled  in  a  suitable  flask  with  10  times  its  volume  of 
water  for  an  hour. 
B — Remove,  yet  warm,  to  a  large  separator  and  set  aside  in  a 
warm  place  for  24  hours ;  the  wash  waters  of  the  flask  are,  of 
course,  added  to  the  contents  of  the  separator. 
C — The  watery  fluid  is  separated  from  the  frothy  top  layer.  This 
layer  is  shaken  out  with  a  large  quantity  (500  cc.)  of  boiling  water 
and  the  separator  again  set  aside  in  a  warm  place  for  24  hours. 
This  operation  is  repeated  until  the  watery  fluid  separates  clear, 
which  takes  usually  3  or  4  days,  and  depends  for  a  good  deal  on  a 
sharp  separation. 
D — After  the  removal  of  the  water,  I  add  100  cc.  of  a  mixture 
of  equal  quantities  of  ether  and  chloroform  (this  is  preferable  to 
chloroform  alone,  which  emulsifies  too  easily,  neither  did  amyl- 
alcohol  yield  good  results).  Agitate  thoroughly,  separate,  collect 
in  a  tared  porcelain  dish,  expel  ether  and  chloroform,  cool  off  and 
weigh  ;  multiply  results  by  4  to  obtain  the  percentage  of  the  oil. 
E — The  oil  is  to  be  examined  in  regard  to  iodine  absorption,  to 
free  and  combined  fatty  acids,  specific  gravity,  iodine,  albumen,  etc., 
just  as  a  natural  product,  this  I  consider  to  be  as  important  as  any 
assay  of  a  crude  drug.  (Compare  on  Standard  Sperm  Oil  in 
Druggists'  Circular^ 
I  determined  lately  the  cod  liver  oil  in  a  few  samples  malt  extract 
with  cod  liver  oil  of  American  manufacture  and  made  an  emulsion 
myself  to  test  the  value  of  the  process  given  above : 
„  Percentage  of  Oil 
JNO-  claimed.  found.  Spec.  Grav. 
*,   40  35  — 
2,   40  26  — 
3,  :  •  12-5  7*5  i"32 
4  (own  made),   12*5  12  1*27 
