Am jjiy ri903arm" }        Epinephrin  and  its  Compounds.  317 
far  as  I  can  now  see,  to  exhibit  all  of  the  reactions  of  monobenzoyl- 
epinephrin  sulphate  as  formerly  described  by  me.  I  would  also 
state  that  very  dilute  solutions  of  this  alkaloidal  sulphate  give  a 
green  color  on  the  addition  of  very  dilute  ammonia,  while  a  similar 
solution  of  the  hydrate,  C10H13NO3^H2O,  takes  on  a  pink  color 
with  the  same  treatment.  Like  the  monobenzoyl  compound,  it  is 
precipitated  from  solutions  of  its  salts  by  ammonia  in  an  amorphous 
form,  easily  soluble  in  excess  of  this  reagent. 
I  would  also  add  that  after  complete  dehydration  the  substance,  in 
the  form  of  the  sulphate  at  least,  has  entirely  lost  its  local  vaso-con- 
stricting  action.  That  it  is  not,  however,  devoid  of  all  physiological 
activity  is  to  be  seen  in  the  work  of  Amberg.1  A  more  complete 
pharmacological  and  therapeutical  study  of  these  alkaloidal  salts  of 
epinephrin  is  now  in  progress  in  my  laboratory. 
I  will  here  offer  the  following  analyses  of  the  simple  alkaloidal 
compound  obtained  by  dehydrating  the  non-alkaloidal  form, 
C10H13NO3^H2O,  with  concentrated  sulphuric  acid.  Specimen  I 
was  twice  precipitated  with  absolute  alcohol,  dried  in  vacuo  over 
sulphuric  acid,  then  in  vacuo  at  65 0  C.  in  order  to  remove  every 
trace  of  alcohol.  Specimen  II  is  like  Specimen  I ;  but  in  conse- 
quence of  an  accident  in  which  concentrated  sulphuric  acid  was 
spilled  into  it,  it  was  again  redissolved  and  reprecipitated  three  times 
in  order  to  remove  all  traces  of  the  acid.  It  was  then  dried  in  vacuo 
over  sulphuric  acid. 
I.  II.  Required  for 
Found  Found.  CCioHi8N08)a  .  H2S04. 
C  =43'9i  C  =  49"°5  C  =49-18 
H=   5-58  H=  5-67  H=  574 
N=   5-99  N=   5-905  N=  574 
H2S04  =  20-59  H2S04  =  20-08 
Analytical  data : 
I.  0*1656  gramme  substance  gave  0*2970  C02  and  0*0831  gramme  H20  ;  there, 
fore,  C  =  48*91  per  cent,  and  H  =  5*58  per  cent. 
0*3895  gramme  gave  20*55  c-c-  of  dry  N  (collected  over  50  per  cent.  KOH) 
at  240  C.  and  751  mm.  barometric  pressure  ;  therefore,  N  =  5*99  per  cent. 
II.  0*2079  gramme  substance  gave  0*3739  gramme  C02  and  0*1060  gramme  H20  ; 
therefore,  C  =  49*05  per  cent,  and  H  =  5*67  per  cent. 
0*4077  gramme  substance  gave  21*1  c.c.  of  dry  N  (collected  over  50  per 
cent.  KOH)  at  230  C.  and  752  mm.  barometric  pressure  ;  therefore,  N  =  5*95 
per  cent. 
1  Archives  internat.  de  Pharmacodynamic  et  de  Therapie,  Vol.  XI,  1902, 
P-  79- 
