302 
ON  ANEMONE  LUDOVICTANA. 
sugar,  with  liquor  potassse  and  sulphate  of  copper.  Sesquichlo- 
ride  of  iron  produced  the  deep  greenish  black  color  characteris- 
tic of  tannic  acid.  A  small  proportion  of  gallic  acid  was  also 
ascertained  to  be  present. 
The  residue  which  had  been  left  on  the  filter  was  removed 
and  triturated  with  ether,  thrown  on  a  filter  and  washed  with 
ether.  The  evaporation  of  this  ethereal  solution  yielded  a  very 
small  amount  of  oily  resinous  matter,  which  did  not  leave  a 
greasy  stain  on  heated  paper,  but  rather  made  the  spot  more 
brittle  and  rougher  than  it  was  previously. 
The  portion  insoluble  in  alcohol  and  ether  was  then  treated 
with  water,  forming  a  dark  brown  solution,  which  was  evapo- 
rated to  a  syrupy  consistence  having  a  black  color.  This  gave 
yellowish  brown  precipitates  with  acetate  and  subacetate  of 
lead,  but  none  with  subacetate  after  the  action  of  neutral  ace- 
tate. It  was  evaporated  to  three  grains  of  a  dark  brown,  brit- 
tle, shining,  extractive  matter,  insoluble  in  hot  alcohol,  and  but 
partially  soluble  in  water. 
Experiments  were  next  made  upon  the  dry  flowers,  and  the 
seeds  which  happened  to  have  been  gathered  with  them.  By  a 
comparison  of  weights  in  the  recent  and  in  the  dried  state,  it 
was  found  that  by  exsiccation  1000  parts  had  become  reduced 
to  114-29,  showing  a  loss  of  about  85  per  cent.  They  were  di- 
vided as  finely  as  possible  by  a  cutting  knife,  their  villous  and 
lanuginous  character  precluding  other  modes  of  comminution. 
1000  grains  were  then  digested  with  7  ounces  of  95  per  cent 
alcohol,  expressed  after  four  hours,  and  twice  consecutively  sub- 
jected to  the  same  process.  The  tincture  gave  with  sesquichlo- 
ride  of  iron,  a  greenish  black  color;  with  solution  of  sulphate  of 
quinia,  a  white  precipitate ;  with  solution  of  gelatin,  a  dense, 
white,  flocculent  deposit,  indicative  of  tannic  acid.  Boiled  with 
sulphate  of  copper  and  liquor  potassse,  a  large  amount  of  sub- 
oxide of  copper  was  thrown  down  by  the  grape  sugar  present. 
The  mixed  alcoholic  tinctures  on  evaporation  left  a  greenish 
brown  resin  weighing  110  grains,  and  a  small  amount  of  light 
green  wax-like  matter  clinging  to  the  sides  of  the  vessel. 
As  the  resin  did  not  appear  to  be  strictly  homogeneous,  it  was 
washed  with  successive  portions  of  ether  as  long  as  any  color 
was  communicated  to  that  solvent ;  by  this  operation  all  the 
