ON  ANEMONE  LUDOVICIANA. 
303 
waxy  matter  was  removed,  and  obtained  separately  on  the  evap- 
oration of  its  ethereal  solution.  The  resin  insoluble  in  ether 
was  of  a  firm  consistence,  dark  brown  color,  weighing  104 
grains. 
After  the  llowers  had  been  treated  with  alcohol  for  the  third 
time,  they  were  dried,  and  found  to  weigh  870  grains,  having 
lost  13  per  cent  of  alcoholic  extract.  They  were  then  covered 
with  water  and  digested  in  a  steam  bath  for  two  hours.  The 
expressed  liquid  gave  dense  precipitates  with  acetate  and  sub- 
acetate  of  lead  used  successively,  thereby  showing  the  presence 
of  gum  and  allied  bodies. 
120  grains  of  the  flowers  finely  cut  up  were  then  arranged  in 
a  percolator  and  exhausted  with  ether.  After  the  menstruum 
had  evaporated  spontaneously,  there  was  left  a  greenish  resin- 
ous fluid.  On  the  application  of  heat  this  separated  into  a  hard 
resin,  disposed  to  cohere  and  form  small  lumps,  which  could 
easily  be  removed  mechanically,  and  a  small  amount  of  fluid, 
light  colored,  translucent,  oily  matter,  possessing  a  very  acrid 
and  almost  caustic  taste.  This  was  volatilized  at  a  more  ele- 
vated temperature,  producing  a  very  irritating  pungent  vapor, 
affecting  the  eyes  and  causing  sneezing.  The  hard  resin  was 
also  possessed  of  a  peculiar  taste,  though  it  differed  essentially 
from  that  of  the  other;  it  was  found  to  be  insoluble  in  strong 
alcohol. 
The  flowers,  after  being  treated  with  ether,  were  displaced 
with  strong  alcohol,  as  long  as  any  color  was  imparted  to  the 
rnenstruurm  The  evaporation  of  this  liquid  yielded  a  resin  in- 
soluble in  ether,  analogous  to  that  which  had  been  obtained  from 
the  first  lot  of  flowers,  besides  several  drops  of  a  brown,  ropy 
solution,  which,  judging  from  its  taste,  appeared  to  contain,  sim- 
ply grape  sugar. 
When  the  same  flowers,  already  extracted  by  ether  and  alco- 
hol, were  exhausted  with  cold  water,  and  this  evaporated,  a  brit- 
tle, brown  extractive  matter  was  procured,  having  but  little 
taste  and  odor.  The  residue  of  these  operations  was  treated 
with  strong  sulphuric  acid,  being  completely  charred  and  made 
miscible  with  water. 
An  alcoholic  extract  prepared  from  a  fresh  portion  of  the 
flowers  according  to  the  directions  of  several  of  the  Pharmaco- 
