54 
L asiosip lion  Mc iss iicrianus. 
{Am.  Jour.  Pharm. 
Felauary,  1911. 
substance  was  obtained,  which  separated  from  alcohol  in  plates,  and 
gave  the  color  reaction  of  the  phytosterols.  On  recrystallizing  the 
substance  from  a  mixture  of  ethyl  acetate  and  dilute  alcohol  it  was 
obtained  in  the  form  of  flat  needles,  melting  at  132-133°. 
0.1188,  when  dried  at  110°,  lost  0.0056  H.O.    H,0  =  4.7 
0.1 132  of  anli3'droiis  substance  gave  0.3468  CO2  and  0.1250  H2O. 
C  =  83.5  ;H=  12.2 
,  Cj7H4(;0,H20  requires  HuO  =  4.5  per  cent. 
CiiTHinO  requires  €  =  83.9;  H      11. 9  per  cent. 
This  substance  is  thus  seen  to  be  a  phytosterol,  and  a  determina- 
tion of  its  optical  rotatory  power  gave  the  following  result : 
0.2722  of  anhydrous  substance,  made  up  to  25  c.c.  with  chloroform,  gave 
a£) —  0°  40'  in  a  2  dcm.  tube,  whence       —  30.6°. 
The  acetyl  derivative,  when  crystallized  from  acetic  anhydride, 
separated  in  needles  melting  at  110°. 
The  alkaline  liquid  from  which  the  above-described  phytosterol 
had  been  extracted  by  means  of  ether  was  acidified  and  again  ex- 
tracted with  ether,  the  ethereal  liquid  being  dried  and  the  solvent 
removed.  A  quantity  of  fatty  acids  in  the  form  of  a  dark  green  mass 
was  thus  obtained.  These  acids  were  distilled  under  diminished 
pressure,  and,  by  means  of  their  lead  salts,  were  separated  into  solid 
and  liquid  portions.  The  amount  of  solid  acid  obtained  was  3 
grammes.  It  distilled  between  220  and  230° and,  when  re- 
crystallized  from  ethyl  acetate,  melted  at  64°. 
0.1320  gave  0.3634  CO2  and  0.1470  H,0.    C  =  75.1  ;  H  =  12.4 
Ci.iHs^Oo  requires  C  =  75.o;  H  =  12.5  per  cent. 
The  solid  acid  thus  appeared  to  consist  of  nearly  pure  palmitic 
acid. 
The  liquid  acids  distilled  between  215  and  22^°/^-^^.  and 
amounted  to  2.5  grammes.  Determinations  of  the  iodine  and  neu- 
tralization values  gave  the  following  results : 
0.2276  absorl)ed  0.2308  iodine.    Iodine  value  =  101.4 
0.2034  neutralized  0.4025  KOH.    Neutralization  value  =197.9 
Ci.sH.'!402  requires  Iodine  value  =  go.o  ;  Neutralization  value  =  198.9 
These  results  indicated  that  the  liquid  acids  consisted  chiefly  of 
oleic  acid,  with  a  very  small  amount  of  an  acid  of  a  higher  degree  of 
unsaturation. 
