'''"dctobe;,i9iT-}<^«'^  Method  for  Testing  Digitalis,  Etc.  481 
minutes,  having  received  almost  the  exact  theoretical  amount.  To 
accurately  determine  the  effect  of  artificial  respiration  upon  the 
lethal  dose  would  of  course  require  a  large  number  of  experiments, 
an  interesting  point,  but  one  I  have  not  been  able  to  work  out 
for  lack  of  time  and  animals. 
Ouabain. 
Date 
Cat  No. 
VV  b,    ill  XV^llJ-b. 
Ouabain  in 
Gms.  perKgm. 
Time 
in  Min, 
I2-16-IO  . 
.  .  .  I 
Male 
2.10 
25.0 
.000,119 
99 
I2-18-IO  . 
.  .  .  2 
Male 
4.70 
32.0 
.000,068 
70 
I2-2I-I0  . 
3 
Male 
2.80 
25.0 
.000,089 
71 
12-22-10  . 
.  . .  4' 
Male 
2.00 
15.0 
.000,075 
75 
12-24-10  . 
•  •  •  5 
Female 
1. 10 
11-5 
.000,104 
118 
12-24-10  . 
...  6 
Male 
1.80 
16.8 
.000,093 
87 
I-  4-1 I  . 
•  •  •  7 
Male 
1.70 
16.0 
.000,094 
93 
I-         I  • 
.. .  8 
Male 
2.70 
16.0 
.000,060 
61 
I-30-II  . 
.. .  9 
Female 
1. 19 
II. 2 
.000,094 
88 
I-3I-II  . 
. . .  10 
Male 
2. II 
22.1 
.000,105 
94 
2-  I-II  . 
. . .  II 
Female 
1.77 
174 
.000,098 
95 
2-  2-1 I  . 
. . .  12 
Female 
2.33 
19.6 
.000,084 
82 
2-  2-1 I  . 
...  13 
Female 
0.97 
13.0 
.000,134 
104 
2-  3-1 I  . 
...  T4 
Female 
2.13 
27.0 
.000,126 
106 
2-  4-1 1  . 
...15 
Male 
342 
46.0 
.000,134 
106 
2-  4-1 I  . 
...16 
Female 
2.43 
26.5 
.000,109 
97 
2-I0-II  . 
. .  .  17 
Male 
2.50 
23.0 
.000,092 
82 
2-1 I-II  , 
...18 
Male 
3.27 
32.7 
.000,100 
91 
2-I3-II  . 
...19 
Male 
2.94 
25-5 
.000,086 
77 
2-I3-II  . 
. . .  20 
Male 
1.81 
17-5 
.000,096 
86 
2-I4-I I  . 
. .  .  21 
Male 
2.40 
20.0 
.000,083 
76 
2-I4-II  . 
. . .  22 
Female 
1.87 
16.0 
.000,085 
83 
2-I5-II  . 
...23 
Male 
2.28 
22.0 
.000,096 
90 
2-1 5-1 I  . 
...24 
Female 
2.25 
21.0 
.000,093 
87 
2-1 5-1 I  . 
...25 
Female 
1.92 
13-5 
.000,070 
65 
6-I4-II  . 
...26 
Female 
2.38 
24.0 
.000,100 
87 
1  Received  paraldehyde  instead  of  ether. 
Two  samples  of  strophanthus  seed  (Koml)e)  were  received  for 
testing  at  this  time.  These  were  reduced  to  No.  60  powder.  Ten 
gram  samples  were  placed  in  150  c.c.  Erlenmeyer  flasks,  supplied  with 
good,  tightly  fitting  corks,  and  macerated  with  40  c.c.  of  75  per 
cent,  alcohol  for  72  hours  with  occasional  agitation.  The  content 
of  each  flask  was  then  poured  into  a  small  narrow  percolator  fitted 
at  the  neck  with  a  tight  pluget  of  cotton.  The  first  ])ortion  of  each 
percolate  was  returned  and  the  percolation  was  then  allowed  to  i)ro- 
