482  Cat  Mctlwd  for  Tcstiiio-  Di<:italis  Etc  i  Am.  jour.  Pharm. 
^  *  (     October,  1911. 
ceed  at  the  rate  of  10  drops  per  minute.  Seventv-five  per  cent, 
alcohol  was  added  from  time  to  time  until  200  c.c.  of  percolate  were 
obtained,  thus  finishing  a  5  per  cent,  tincture.  For  injection,  i  :  6000 
solutions  were  used.  These  were  made  in  the  same  manner  as 
described  under  ouabain.  The  results  from  eight  experiments  on 
each  of  these  samples  were  as  follows  : 
Strophanthus  Seed  No.  B-565. 
Dilution  i:6ooo  i  c.c.  =  .000,166  gm.  drug. 
Date 
Cat  No. 
Sex 
Wt.  in  Kgms. 
c.c.  Sol. 
Strophanthus 
in  Gm. 
Time 
2- I 6-1 I 
.  .  .  .  I 
Male 
2.23 
16.0 
.001,19 
69 
2-16-II 
Male 
2.65 
22.0 
.001,37 
100 
2-16-II  . 
■  ■■■  3 
Female 
2.82 
26.0 
•001,53 
98 
2-1 7-1 1 
. .  . .  4 
Male 
2.29 
22.9 
.001,66 
107 
2-1 7-1 I  , 
....  5 
Male 
2.II 
24.0 
.001,88 
100 
2-18-II  , 
,  ,  6 
Male 
2.98 
30.0 
.001,66 
95 
2-18-I I  , 
.. ..  7 
Male 
3-07 
3I-0 
.001,67 
100 
2-18-II 
8 
Female 
2.04 
18.0 
.001,46 
66 
Strophanthus 
Seed  No. 
B-566. 
2-21-11    ,  , 
I 
Male 
2.45 
26.4 
.001,78 
102 
2-21-11 
Male 
3-17 
23.0 
.001,20 
60 
2-21-11  , 
•  •  3 
Male 
1.29 
II. I 
.001,42 
71 
2-22-1 I  , 
•  •  4 
Male 
3.00 
24.0 
.001,32 
60 
2-22-1 1 
5 
Male 
3-17 
25-5 
•001,33 
87 
2-22-1 I 
6 
Female 
3-14 
235 
.001,23 
87 
2-23-1 1    . .  , 
•  •  7 
Male 
3.82 
29.0 
.001,24 
95 
2-23-11   .  , . 
8 
Male 
2.93 
25.0 
.001,41 
III 
Hatcher  and  Brody  have  found  after  many  experiments  that  if 
digitalis  and  the  other  members  of  the  series  are  injected,  like 
ouabain  and  strophanthus,  until  the  animal  dies,  the  results  will 
usually  be  too  high — necessitating  a  correction  of  about  20  per  cent. 
They  have  therefore  devised  a  modification  of  the  method  which 
gives  results  comparable  in  accuracy,  they  believe,  to  those  obtained 
with  crystalline  ouabain  itself.    This  modification  is  as  follows : 
A  measured  quantity  of  the  digitalis  solution  (I  understand  about 
50  per  cent,  of  the  required  amount)  is  injected  during  the  first 
period  of  about  ten  minutes.  After  an  interval  of  about  twenty 
minutes  the  injection  is  continued,  substituting  ouabain  solution  for 
the  digitalis,  until  the  animal  dies.    The  difference  between  the 
