556  Active  Principle  of  Suprarenal  Gland.  |  ^S;:cSIHr^^9T■ 
The  solution  of  the  purified  base  used  in  these  experiments  either 
was  made  up  fresh  every  day  or  it  was  made  up  by  diluting  a  stock 
1  to  10,000  solution  which  was  kept  on  ice  to  prevent  deterioration. 
Dilutions  of  i  to  50,000  and  i  to  100,000  were  used  and  the  injec- 
tion of  both  the  known  and  unknown  solutions  varied  from  0.5  to 
i.o  ex. 
The  injections  might  possibly  all  be  made  through  one  cannula, 
but  to  obviate  the  necessity  of  washing  out  the  residual  solution  each 
time,  cannulse  are  placed  in  the  right  and  left  femoral  vein,  one  for 
injecting  the  known  and  the  other  the  unknown  solution.  As 
injections  made  at  different  rates  produce  different  results  it  is 
necessary  to  make  both  injections  at  as  nearly  the  same  rate  and  as 
rapidly  as  possible. 
Two  tables  are  given  to  show  the  method  of  arriving  at  the  assay 
value  of  a  given  product.. 
Table  II. 
Sample  desiccated  suprarenal  glands,  No.  363,  dog  7.6  kg.  body  weight ; 
morphine  sulphate  0.015  gm.  per  kg.  subcutaneously.  Both  vagi  cut.  Epine- 
phrine base  in  i  to  100,000  acidulated  Ringer  solution.  No.  363  suprarenal 
gland  I  mg.  to  i  c.c.  acidulated  Ringer  solution. 
Blood 
Blood 
Rise 
pressure 
pressure 
in 
Time. 
Preparation. 
Dose. 
before. 
after. 
millimeters. 
11.48 
Epinephrine 
0.5  c.c. 
166 
206 
40 
II.51 
363 
0.5  mg, 
164 
204 
40 
11.56 
363 
0.5  " 
162 
200 
38 
12.02 
Epinephrine 
0.4  c.c. 
156 
192 
36 
12.05 
363 
0.4  mg. 
156 
192 
36 
12.12 
Curare 
12.17 
Epinephrine 
0.6  c.c. 
152 
188 
36 
12.19 
363 
0.6  mg. 
148 
182 
34 
12.23 
363 
1.0  " 
144 
190 
46 
12.26 
Epinephrine 
1.0  c.c 
138 
182 
44 
12.29 
363 
1.0  mg. 
140 
186 
46 
It  is  easily  seen  from  the  above  record  that  i  milligram  of  the 
desiccated  gland  gave  the  same  rise  as  i  c.c.  of  a  i  to  100,000  epine- 
phrine solution,  or  0.0 1  milligram  estimated  as  the  base.  The  desic- 
cated gland  therefore  contained  i  per  cent,  of  the  b'lse. 
Table  III  further  illustrates  the  method  of  arriving  at  the  assay 
value. 
