166  New  Agent  in  Pulmonary  Tuberculosis.  {Am-/^-^Tm' 
may  be  continued  indefinitely  day  after  day  without  producing 
symptoms  of  renal  irritation.  It  is  antiseptic  and  bactericidal  when 
applied  to  cutaneous  and  mucous  surfaces. 
Antiseptic  and  Germicidal  Properties  of  Oxypinene. — From  pure 
broth  cultures  of  typhoid,  diphtheria,  and  colon  bacilli,  and  staphy- 
lococcus, smears  were  made  on  cover-slips.  These  were  thoroughly 
dried  in  the  incubator,  and  placed  under  a  bell-jar,  into  which 
oxypinene  was  allowed  to  enter  through  an  opening  near  its  base, 
and  escape  through  an  opening  at  the  top  which  was  lightly  plugged 
with  cotton.  At  intervals  of  forty  minutes,  one  hour,  one  and  a 
half  hours,  and  two  hours,  a  smear  of  each  culture  was  removed  and 
dropped  into  broth  tubes.  After  incubation  all  the  controls  except 
that  of  diphtheria  showed  growth.  All  exposed  smears  produced 
growth  except  those  of  diphtheria. 
The  same  organisms  were  used,  but  instead  of  incubator-dried 
smears,  large  drops  of  broth  cultures  of  each  on  cover-slips  were 
exposed,  in  the  same  manner  as  previously,  for  fifty  minutes,  seventy 
minutes,  and  one  hour  and  fifty  minutes.  After  incubation,  all 
controls  showed  marked  growth.  All  exposed  drops  failed  to  produce 
growth,  except  that  of  staphylococcus,  growth  of  which  was  markedly 
retarded  and  very  slight.  As  all  the  exposed  drops  became  dry  before 
the  expiration  of  forty  minutes,  and  as  in  the  previous  experiment 
none  of  the  incubator-dried  smears  were  killed,  it  was  concluded 
either  that  the  organisms  in  all  exposed  drops  were  killed  under 
forty  minutes,  or  that  at  least  some  moisture  is  necessary  to  obtain  a 
germicidal  effect.  As  on  theoretical  grounds  the  latter  supposition 
seemed  probable,  the  use  of  incubator-dried  preparations  was 
abandoned. 
Agar-agar,  in  Petri  plates,  was  substituted  for  cover-slips,  and 
upon  the  surfaces  of  these,  broth  cultures  of  the  organisms  already 
used  were  planted  in  streaks.  These  plates  were  then  exposed  in  the 
manner  already  described  for  one  and  one-half  hours.  All  controls 
showed  marked  growth  after  twenty-four  hours'  incubation.  All 
exposed  plates  produced  no  growth  after  five  days'  incubation. 
Large  drops  of  broth  culture  of  the  typhoid  bacillus,  after  ex- 
posure on  cover-slips  in  the  manner  described,  for  twenty-five  and 
forty  minutes,  were  smeared  upon  agar-agar  slant  tubes.  The  con- 
trols showed  marked  growth  after  eighteen  hours'  incubation.  The 
tubes  inoculated  with  culture  which  had  been  exposed  for  twenty- 
five  minutes  showed  marked  growth  after  twenty-four  hours'  incuba- 
