AmApriir'i9i5rm'}^^w  -4gent  in  Pulmonary  Tuberculosis.  167 
tion.  The  tubes  inoculated  with  culture  which  had  been  exposed 
for  forty  minutes  showed  no  growth  after  ninety-six  hours' 
incubation. 
Large  drops  of  broth  culture  of  each  of  the  organisms  previously 
used  were  exposed  in  the  same  manner  and  for  the  same  periods  as 
in  the  preceding  experiment,  and  planted  on  agar-agar  slant  tubes. 
The  controls  showed  marked  growth  after  eighteen  hours'  incubation. 
The  tubes  inoculated  with  culture  which  had  been  exposed  for 
twenty-five  minutes  showed  growth  after  twenty-four  hours'  incuba- 
tion. The  tubes  inoculated  with  culture  which  had  been  exposed 
for  forty  minutes  showed  no  growth  after  ninety-six  hours' 
incubation. 
The  conclusion  seemed  to  be  justified  that  for  typhoid,  diphtheria, 
and  colon  bacilli,  and  the  staphylococcus,  in  pure  broth  culture,  when 
even  the  slightest  moisture  is  present,  oxypinene  is  germicidal  in 
from  twenty-five  to  forty  minutes. 
Upon  the  surface  of  agar-agar,  in  Petri  plates,  broth  cultures  of 
subtilis,  anthrax,  and  pneumococcus  were  planted  in  streaks.  These 
plates  were  exposed  in  the  manner  already  described  for  one  and 
one-half  hours.  The  controls  showed  marked  growth  after  eighteen 
hours'  incubation.  The  exposed  plates  showed  no  growth  after  forty- 
eight  hours'  incubation.  At  the  end  of  five  days  no  growth  had 
appeared. 
Upon  the  surface  of  agar-agar,  in  Petri  plates,  broth  cultures  of 
typhoid,  diphtheria,  and  anthrax  bacilli,  and  staphylococcus  were 
planted  in  streaks.  These  plates  were  exposed  for  two  and  one-half 
hours,  not  under  the  bell- jar,  but  in  a  room  having  a  cubic  capacity 
of  about  2500  feet.  The  room  was  fairly  well  filled  with  oxypinene. 
The  controls  showed  marked  growth  after  eighteen  hours'  incuba- 
tion. The  exposed  plates,  after  twenty-four  hours'  incubation, 
showed  no  growth,  except  that  of  staphylococcus,  on  which  a  very 
slight  growth  appeared.  After  forty-eight  hours'  incubation,  anthrax 
seemed  to  show  slight  growth,  but  at  the  end  of  seventy-two  hours 
it  had  not  spread,  and  a  smear  of  it  planted  on  an  agar  slant  tube 
failed  to  produce  growth.  A  single  agar  Petri  plate,  on  the  sur- 
face of  which  the  same  organisms,  in  broth  culture,  had  been 
streaked,  was  exposed  under  the  bell-jar  for  two  hours,  and  failed  to 
show  growth  of  any  organism,  after  seventy-two  hours'  incubation, 
while  anthrax  overgrew  all  other  growths,  on  the  control  plate,  after 
twenty-eight  hours'  incubation. 
