The  Examination  of  Chaulmoogra  Oil.  |A o^ber 
Pharm. 
er,  1915. 
(H)  Saponification  Value  of  Acetylated  Oil. — This  value 
is  also  known  as  "  Benedikt's  acetyl  acid  value,"  and  was  obtained 
by  determining  the  saponification  value  of  the  acetylated  oil  with 
phenolpthaline  as  indicator. 
(1)  Smith  Oil. — 14856  grammes  acetylated  oil  taken.  After 
saponification  11.1  Cc.  semi-normal  acid  were  required  to  neutralize 
the  excess  of  alkali.    Therefore  the  Benedikt  value  = 
(33-5T48y°8-o6= 4*3.07. 
(2)  B.  K.  Paul  Oil. — 1.9664  grammes  of  acetylated  oil  taken. 
14.7  Cc.  standard  acid  was  required.    Therefore  Benedikt  value  = 
18-8X28-06  =  527-828  =  26g2> 
1-9664  1-9660 
The  high  Benedikt  value  shows  the  presence  of  triglycerides  in 
Smith  oil  ^and  confirms  the  previous  statement. 
(/)  Ester  Value. — This  is  the  difference  between  acid  value 
and  saponification  value  of  the  oil.  In  case  of  Smith  oil  it  is  204.26, 
and  in  case  of  B.  K.  Paul  oil  it  is  129.27. 
The  great  difference  between  the  ester  value  of  the  two  oils  once 
more  confirms  the  view  formerly  expressed  that  Smith  oil  consists 
almost  wholly  of  neutral  ester  (triglycerides),  whereas  the  other 
oil  is  a  mixture  of  tri-  and  di-  glycerides. 
N.  B. — The  ester  value  of  the  chaulmoogra  fats  was  110.6. 
Examination  of  Fatty  Acids.  - 
One  hundred  grammes  of  the  oil  were  saponified  with  the  cal- 
culated quantity  of  caustic  potash  dissolved  in  a  mixture  of  100  Cc. 
water  and  50  Cc.  alcohol  and  boiling  under  a  reflux  condenser.  The 
fatty  acids  were  then  liberated  by  heating  with  excess  of  dilute 
sulphuric  acid.  The  melted  fatty  acids  floated  on  the  surface  of 
the  water  as  an  oil.  After  washing  and  cooling,  both  the  acids 
were  obtained  in  a  white,  crystalline  mass  and  had  a  sweet  odor 
suggesting  that  of  the  acid  obtained  from  cocoanut  oil.  They  could 
not  be  distinguished  from  one  another  by  color,  odor,  or  appearance. 
(I)  The  fatty  acids  from  Smith  oil  gave  solidifying  point  by 
titre  test  at  36.5 0  C.  The  acid  was  recrystallized  once  from  alcohol 
and  then  from  benzene  and  the  solidifying  point  again  determined. 
It  was  found  to  be  370  C.  The  melting-point  was  the  same  as  the 
solidifying  point. 
