AmM°aU/;il9h8arm-}  Cascarilla  Bark.  239 
(1)  0*215  of  platinum  compound  left  on  ignition  0*065  of  platinum. 
(2)  0*335  "  "  "  "  o*ioo 
(3)  0*120  "  "  "  "  0*0365 
(1)  (2)  (3)  Theory  of  Betaiue.    Theory  for  Choline. 
30-23  29-85         30-41  30-28  31-65 
Mean,  30*16  per  cent,  of  platinum. 
That  the  chloride  of  this  base  yields  on  ignition  trimethylamine, 
indicates  that  it  is  allied  to  choline.  That  its  chloride  is  practically 
insoluble  in  alcohol  and  melts  with  intumescence  on  heating,  and 
that  its  platinum  salt  yields  a  mean  of  30*16  per  cent,  of  platinum, 
prove  beyond  a  doubt  that  the  base  in  question  is  not  choline,  but 
betaine. 
CHLOROFORMIC  RESIDUE. 
It  was  treated  with  warm  hydrochloric  acid,  3  per  cent.,  the  filtered 
solution  was  made  alkaline  with  ammonia  and  then  shaken  with 
chloroform.  After  evaporation  of  the  chloroform  the  residue  was 
taken  up  with  the  weak  acid,  and,  after  the  addition  of  ammonia, 
was  again  shaken  with  chloroform.  The  product,  which  was  not 
quite  free  from  color,  was  alkaline  and  soluble  in  alcohol,  ether  and 
chloroform.  It  contained  nitrogen.  A  solution  of  a  portion  of  it 
in  weak  acid  was  precipitated  by  ammonia,  also  by  iodine  and  potas- 
sium iodide,  Mayer's  reagent,  Thresh's  reagent,  cadmium  and  potas- 
sium iodide,  and  phosphomolybdate  of  sodium.  To  the  solution  of 
another  portion  in  weak  acid  the  addition  of  platinic  chloride  gave 
a  buff-colored  precipitate,  which  was  collected  and  washed  free 
from  platinum  chloride.  When  air-dried  it  was  soluble  in  alcohol 
and  crj'stallized  from  hot  water  in  prismatic  plates.  This  alkaloidal 
substance  was  also  obtained  from  the  impure  cascarillin  yielded  by 
Alessandri's  process.  The  existence  of  a  base  in  cascarilla  bark 
other  than  one  allied  to  choline  has  been  a  debatable  point,  but 
may  now  be  accepted  as  a  fact.  It  is  believed  that  this  is  the  first 
time  that  the  alkaloid  cascarilline  has  been  isolated  and  its  platinum 
compound  prepared. 
My  thanks  are  due  to  Mr.  John  J.  Bryant  for  his  assistance  in 
carrying  out  the  operations  described  in  this  paper. 
