56 
Japanese  Lac. 
f  Am.  Jour.  Pharru. 
\    February,  1906. 
alcoholic  extract  consisted  of  a  mixture  of  two  or  more  substances. 
But  in  no  case  can  the  above  method  be  considered  as  a  complete 
separation.  Alcoholic  solutions  of  each  of  the  fractions  assumed  a 
green  or  greenish-black  color  with  alkalies,  the  color  varying  with 
the  concentration  of  the  solution  and  the  strength  of  the  alkali. 
By  shaking  an  ether  solution  of  resinous  substances  with  a  I  per 
cent,  solution  of  sodium  carbonate  a  solution  was  obtained  which 
deposited  a  reddish-brown  precipitate  on  the  addition  of  an  acid. 
This  precipitate  was  insoluble  in  all  ordinary  solvents,  including 
acids  and  alkalies,  except  fuming  nitric  acid.  This  is  doubtless  the 
same  substance  which  Yoshida  obtained  and  named  "  Oxyurushic 
acid,"  but  as  it  does  not  possess  any  of  the  properties  of  an  acid 
Professor  Tschirch  suggested  the  name  "  Oxyurushin,"  which  I  have 
adopted.  All  of  the  resinous  substances  may  be  converted  into 
oxyurushin  by  heating  with  a  sufficient  quantity  of  fixed  alkali, 
when  it  will  be  precipitated  as  a  black  insoluble  compound,  changing 
to  reddish  brown  on  the  addition  of  acids. 
Separation  into  Benzin-soluble  and  Benzin-insoluble. — At  first  it  ap- 
peared as  though  the  alcoholic  residue  was  soluble  in  all  of  the 
ordinary  solvents  for  oils  and  resins,  but  investigation  proved  that  it 
was  not  completely  soluble  in  all  proportions  of  carbon  disulphide, 
methyl  alcohol,  amyl  alcohol  or  petroleum  benzin.  After  numerous 
experiments  the  following  method  was  adopted:  One  part  of  the 
resinous  substance  was  dissolved  in  7  parts  of  benzin,  forming  a 
clear  solution,  but  further  addition  of  benzin  caused  a  precipitate. 
This  was  then  poured  into  55  parts  of  benzin,  which  caused  an  im- 
mediate separation  of  a  thick,  brown  mass,  leaving  a  cloudy  liquid. 
After  twelve  hours  the  benzin  became  clear  and  was  not  affected  by 
the  further  addition  of  benzin.  The  benzin  was  decanted  and  the 
precipitate  dissolved  in  a  small  quantity  of  benzin  and  again  pre- 
cipitated by  pouring  into  a  larger  amount.  This  operation  was 
repeated  several  times  until  the  precipitate  became  entirely  insolu- 
ble in  benzin.  By  this  method  the  resinous  substances  were  sepa- 
rated into  a  benzin-soluble  and  a  benzin-insoluble. 
Separation  of  the  Benzin-insoluble  into  Methyl  Alcohol- soluble  and 
Methyl  Alcohol-insoluble. —  By  treating  the  benzin-insoluble  portion 
with  methyl  alcohol  it  could  be  separated  into  two  substances,  one 
soluble  and  the  other  insoluble  in  methyl  alcohol.  Only  about  half 
of  the  insoluble  portion  was  soluble  in  ether,  the  remainder  appar- 
