Am.  Jour.  Pharm. ) 
February,  1906.  J 
Japanese  Lac. 
57 
ently  having  undergone  oxidation  into  oxyurushin  during  manipula- 
tion with  methyl  alcohol  and  evaporation.  This  theory  is  supported 
by  the  fact  that  the  methyl  alcohol  solution  becomes  cloudy  on 
standing.  A  similar  change  doubtless  takes  place  in  ethyl  alcoholic 
solutions,  though  much  more  slowly,  as  a  slight  deposit  was  noticed 
when  an  alcoholic  solution  was  allowed  to  stand  for  some  time. 
Separation  of  the  Benzin-soluble  into  two  or  more  substances. — The 
benzin  solution  was  evaporated  and  I  volume  of  the  oily  residue 
dissolved  in  8  volumes  of  benzin  and  4  volumes  of  alcohol  added 
and  the  whole  thoroughly  agitated.  Upon  standing  it  separated 
into  two  layers.  The  upper  benzin  layer  was  of  a  yellowish-brown 
color,  the  lower  alcohol  layer  reddish  brown.  These  were  sepa- 
rated and  the  benzin  solution  washed  with  alcohol  as  long  as  the 
washings  were  colored.  The  benzin  was  evaporated,  leaving  an  oily, 
non-poisonous,  brown  residue.  The  alcoholic  solution  was  evapo- 
rated, leaving  a  slightly  gelatinous  residue,  and  by  rapidly  washing 
this  with  benzin  a  small  quantity  of  oily  residue  was  separated  from 
the  gelatinous  mass.  Both  were  poisonous.  Further  washing  with 
benzin  dissolved  the  gelatinous  portion.  I  believe  that  the  alcoholic 
portion  consists  of  a  poisonous  and  a  non-poisonous  substance,  but 
thus  far  have  not  been  able  to  make  a  complete  separation.  I  hope 
to  do  this  later. 
All  of  the  above  substances  in  alcoholic  solutions  were  precipitated 
by  lead  acetate,  subacetate,  silver  nitrate,  mercurous  nitrate,  cupric 
acetate  and  ferric  chloride.  The  lead  precipitates  were  of  a  light- 
gray  color,  gradually  becoming  darker  on  standing.  All  the  other 
precipitates  were  black. 
The  resins  insoluble  in  benzin,  on  exposure  to  the  air  in  thin  layers, 
slowly  changed  to  oxyurushin.  Numerous  combustions  were  made 
of  these  and  also  of  the  oxyurushin  formed  by  the  action  of  alkali. 
The  oxidation  products  seem  to  be  the  same  in  all  cases.  The  mean 
of  the  results  of  combustion  is  as  follows  : 
Found. 
72-137 
8-I58 
1-652 
l8'053 
Calculated  for 
C10oH1S8NoO10. 
72'206 
8"202 
I-656 
17-936 
The  Gum-enzyme. — After  extracting  the  lac  with  alcohol  the  resi- 
due was  extracted  with  cold  water  and  the  gum-enzyme  precipitated 
