"^"jS;  iSl }     Liquid  cmd  Saccliarated  Pepsins.  5 
it  swells  up  considerably  and  after  some  time  disintegrates  itself  to 
white  flakes,  which  float  at  first  and  then  settle.  Although  easily 
soluble  when  freshly  precipitated,  it  dissolves,  after  being  dried,  very 
little  in  cold  water,  more  in  water  of  80  degrees,  but  very  quickly  by 
addition  of  a  little  acid.  It  is  therefore  necessary,  when  Saccharated 
Pepsin  is  prescribed  in  solution,  to  add  a  little  acid,  hydrochloric  or 
lactic.  To  make  Liquid  Pepsin  from  the  dry  Saccharated  Pepsin, 
I  propose  the  following  formula  : 
R    Sacch.  Pepsin,  64  grains. 
Water,  5  fluid  ounces. 
Hydrochloric  acid,  1  fluid  drachm. 
Shake  in  a  bottle  until  the  milk-sugar  and  Pepsin  are  completely  dis- 
solved, then  add  glycerin  3  fluid  ounces,  and  filter.  A  colorles® 
liquid  is  formed,  of  which  1  fl.  oz.  dissolves  IJ  drachms  of  coagulated 
albumen. 
•  As  the  normal  gastric  juice  of  man  and  animals  contains  chloride 
of  sodium,  I  tried  to  ascertain  if  the  addition  of  a  little  table  salt  to 
a  solution  of  unmixed  Pepsin  in  acidulated  water  would  accelerate 
the  solution  of  coagulated  albumen ;  the  result  was  that  Pepsin 
with  chloride  of  sodium  dissolved  albumen  much  quicker  than  with- 
out it.  I  therefore  mention  here  that  chloride  of  sodium  is  added  to 
the  Saccharated  Pepsin. 
As  for  the  strength  of  Saccharated  Pepsin,  compared  with  the 
other  dry  Pepsins  in  use  here,  it  was  found  that  one  part  of  it 
equalled  about  3J  parts  of  Boudault's,  8  to  9  parts  of  Grimault's, 
12  parts  of  Hawley's  and  at  least,  40  parts  of  Houghton's.  Du- 
ring a  period  of  from  3  to  4  hours,  10  grains  of  Saccharated  Pepsin  in 
a  fluid  ounce  of  water,  acidulated  with  10  drops  of  muriatic  acid  and 
kept  at  a  temperature  of  100  to  105^  Fahrenheit,  dissolved  120  grains 
of  coagulated  albumen.  Under  identical  conditions,  60  grains  of  Bou- 
dault's Pepsin*  dissolved  the  same  amount ;  40  grains  of  Boudault's 
Pepsin  dissolved  the  same  amount ;  30  grains  of  Boudault's  Pepsin  did 
not  quite  dissolve  it;  60  grains  of  Grimault's  Pepsin  dissolved  but  84 
grains  ;  60  grains  of  Hawley's  Pepsin  dissolved  but  60  grains.  With 
*  The  Boudault's  Pepsin,  I  had  used  for  experiments  last  winter,  must  have 
been  adulterated  or  spoiled,  as  I  recollect  right  well  that  it  was  a  damp  sticky 
powder  of  somewhat  different  color  from  the  one  I  used  this  time,  therefore  its 
strength  compared  with  the  Liquid  Pepsin  was  found  so  much  less  than  in 
the  present  experiment. 
