88 
Two  Toxicological  Investigations. 
f  Am.  Jour.  Pharm 
I    February,  1905. 
quantity  of  coloring  matter  associated  with  other  organic  matter, 
so  that  the  method  of  purifying  by  solution  in  ammonia  was  found 
to  be  inapplicable.  It  was  necessary  to  oxidize  it  with  fuming  nitric 
acid,  convert  it  into  a  sodium  salt,  and  reprecipitate  the  sulphide, 
which  was  dried,  washed  with  carbon  disulphide,  petroleum  ether, 
and  finally  dissolved  in  ammonium  hydroxide  and  evaporated  on  a 
tared  watch  crystal.  The  results  indicated  3*93  grains  arsenic  tri- 
oxide  present  in  the  section  of  liver  I  examined. 
The  Kidney. — The  kidney  was  treated  in  the  same  way  as  the 
liver,  and  yielded  1-589  grains  arsenic  trioxide. 
The  Heart —This  was  also  treated  in  the  same  way  as  the  liver, 
and  yielded  0-49  grains  arsenic  trioxide. 
The  Liquid  in  the  Jar. — The  exudation  from  these  organs  remain- 
ing in  the  jar  was  also  extracted,  and  yielded  0-617  grains. 
The  Brain. — The  brain  matter  weighed  750  grammes,  and  was  in 
a  pulpy  and  badly  decomposed  condition  when  received.  It  was  sub- 
jected  to  the  method  of  Fresenius  and  Babo,  and  the  filtered  clear 
solution,  on  treating  with  hydrogen  sulphide,  yielded  a  decided  pre- 
cipitate, which  separated  very  slowly,  and  which  consisted  almost 
entirely  of  organic  matter.  The  quantity  of  arsenic  present  was  so 
small  as  to  yield  only  the  very  faintest  reaction  with  Reinsch's  and 
with  Marsh's  tests,  and  this  trace  was  not  ponderable. 
My  determinations  were  based  upon  the  older  method  of  sepa- 
rating the  arsenic  as  sulphide,  and  yielded  a  total  of  12-454  grains 
of  arsenic  trioxide  from  the  materials  I  examined. 
RESULTS  OBTAINED  BY  PROFESSOR  MOERK. 
Prof.  Frank  X.  Moerk  adopted  a  similar  method  for  the  separa- 
tion of  the  arsenic  from  the  materials  he  analyzed.  He  reported 
that  he  purified  the  sulphide  by  oxidation  with  nitric  acid,  then  dis- 
solved in  ammonia,  and  converted  it  into  magnesium-ammonium 
arsenate,  from  which  the  quantity  of  arsenic  trioxide  was  calculated. 
The  results  were  as  follows  : 
The  kidney  weighed  557*86  grains,  and  yielded  1-471  grains 
As203- 
The  piece  of  intestine  weighed  422-83  grains,  and  yielded  1-147 
grains  As203. 
The  spleen  weighed  339-5  grains,  and  yielded  -656  grains  As2Os. 
