468 
Researches  on  Albumin. 
Am.  Joim  Pharnv. 
Septi,  1879. 
conditions  involved  in  the  determination  of  the  sulphur  and  tyrosin,,  a& 
well  as  the  results  of  elementary  analysis,  thus  : 
Mol. 
C.  H.  N.  O.  S.  weight. 
Experiment,        .       52*57        7"i6         i6'6         2i'8         r'8  — 
Theory,        .       .    5262        7*07        16-62       21*64       175  5,473 
Again,  taking  as  the  basis  of  calculation  the  experiments  made  witb 
5  or  6  parts  of  baryta  at  1800,  the  centesimal  proportions  of  the  pro- 
duct were  : 
N  as  NH3.  BaC03.  BaC204.  H4C,02. 
4*03  i7'6  no  4*6 
which  corresponds  very  fairly  for  5,473  parts  of  albumin  with — 
NH3.  H2C204.  C02.  H4C202. 
16  mols.  4  mols.  3  mols.  4  mols. 
From  these  numbers  the  following  equation  may  be  written,  whichv 
moreover,  would  give  a  fixed  residue  of  99*6  per  cent.  : 
C2J„H387  N65O76S3+60H2O=i6NH3+4C2H2O4+3CO2+4C2H4O2+ 
Deducting  a  molecule  of  tyrosin  from  the  formula  of  the  fixed  resi- 
due, we  have  C212H42iN4sO102,  which  approximates  very  closely  to  an 
expression  of  the  form  CnH2nN02,  differing  from  it  only  by  a  slight 
excess  of  oxygen. 
By  taking  in  this  manner  the  series  of  experiments  made  with  dif- 
ferent proportions  of  baryta  and  at  different  temperatures,  a  number  of 
equations  may  be  written,  in  all  of  which  the  theoretical  composition 
of  the  fixed  residue  corresponds  very  closely  with  that  obtained  experi- 
mentally. 
There  can  be  no  doubt  that  during  the  hydration  of  albumin  as  many 
molecules  of  water  are  assimilated  as  there  are  molecules  of  ammonia 
liberated.  If,  then,  from  the  proposed  formula  for  albumin  we  deduct 
the  ammonia,  carbonic,  oxalic  and  acetic  acids  in  the  proportion  fur- 
nished by  experiment,  less  a  number  of  molecules  of  water  equal  to  the 
number  of  molecules  of  ammonia  (adding  as  many  molecules  of  water 
as  there  are  molecules  of  carbonic  anhydride  produced,  since  the  latter 
probably  results  from  the  decomposition  of  urea)  we  shall  have 
[i6NH3+4C2H304+3C02+4C2H402— i6H20+3H20], 
i 
