78  Leaves  of  Eupatorium  Perfoliatum.  {^'^•^^^'^^^'''^ 
Wax. — This  was  obtained  by  treating  the  residue,  after  the  sejmration 
of  the  enpatorin,  with  stronger  ether,  whereby  a  white  substance  was 
separated  and  crystallized  from  petroleum  spirit,  in  white,  tasteless, 
acicular  crystals. 
Absolute  alcohol  dissolved  a  portion  of  these  which  on  evaporation 
became  yellow,  insoluble  in  95  per  cent,  alcohol  and  melting  at  95°C. 
The  insoluble  portion  was  recrystallized  from  petroleum  spii'it  in 
white  tasteless  crystals,  as  shown  by  the  drawing,  but  which  on  warm- 
ing became  yellowish,  melting  at  145°C.,  not  affected  by  sulphuric  acid  or 
aqueous  and  alcoholic  solution  of  potash,  but  soluble  in  glacial  acetic 
acid,  reprecipitated  on  the  addition  of  water. 
Mr.  Latin  also  obtained  a  crystalline  principle  from  the  ethereal 
extract  of  the  herb,  by  means  of  benzin,  but  speaks  of  it  as  having 
a  low  fusing  point. 
Volatile  Oil. — About  2ft)s  of  the  fresh  leaves  were  distilled  with 
water ;  the  distillate  being  slightly  cloudy  was  shaken  with  petroleum 
spirit,  separated  and  evaporated,  showing  a  very  small  amount  of 
volatile  oil  having  the  odor  of  the  fresh  leaves. 
Summary  of  the  analysis  made  according  to  DragendorfPs  scheme : 
Ash  (potassium,  calcium,  iron,  and  silica)  7.5  per  cent. 
Moisture  9.40  per  cent. 
Petroleum  spirit  extract  : 
Volatile  oil,  0.01  " 
Resin,  0.80 
^       fa  portion  melting  at  95°  C 
t       "                  "  145°C.  2.60  " 
Eupatorin,  (in  small  amount), 
Chlorophyll  and  undetermined  resinous  sub- 
stances,   6.19  " 
Ethereal  extract  : 
Gallic  acid,  ■  1.50 
Resin  and  some  chlorophyll,  6.80  " 
Alcoholic  extract : 
8.30 
Tannic  acid,  5.60 
Undetermined  substances,  3.12     "  8.72 
Aqueous  extract: 
Mucilage  and  sugar, 
20.86 
