232 
Cassia  Marilandica. 
( Aja.  Jour.  Pharm. 
I       May,  18^, 
with  petroleum  ;  of  a  dark  green  color,  resinous  and  possessing  the 
odor  of  the  drug.  Almost  all  of  the  chlorophyll  was  in  this  extract. 
It  was  soluble  in  chlorform  and  benzol,  and  partly  so  in  absolute  al- 
cohol; the  melting  point  was  63°  C.  Water  dissolved  but  0' 03  per 
cent.,  including  a  trace  of  tannin.  To  this  aqueous  solution,  alka- 
loidal  tests  were  applied  to  both  alkaline  and  acid  solutions,  but  with 
no  results.  Absolute  alcohol  dissolved  1*98  per  cent.,  leaving  a  resi- 
due of  0*86  per  cent. 
The  extract  with  absolute  alcohol,  representing  7'40  per  cent.,  -was 
mostly  soluble  in  water ;  the  portion  insoluble  was  weighed  as  phlo- 
baphene.  This  aqueous  solution  was  found  to  contain  0*625  per  cent, 
of  tannin,  and  0"558  per  cent,  of  glucose.  The  tannin  was  estimated 
by  precipitation  with  acetate  of  lead,  and  also  with  acetate  of  copper ; 
both  results  varied  only  slightly,  showing  the  presence  of  tannic  acid 
alone.  The  solution  after  precipitation  with  lead  acetate,  and  decom- 
posing the  excess  of  lead  with  HgS  gas,  was  treated  with  Fehling's 
solution,  and  the  amount  of  glucose  determined  gravimetrically.  The 
aqueous  solution  was  also  tested  for  alkaloids  by  agitating  successively 
with  petroleum  spirit,  benzol  and  chloroform,  both  in  alkaline  and 
acid  solutions,  but  with  negative  results  in  all  experiments. 
The  water  extract,  after  deducting  the  ash  (3.60  per  cent.),  amounted 
to  20.24  per  cent.,  was  of  dark-brown  color,  and  had  the  odor  of  burnt 
sugar.  The  mucilage  was  precipitated  with  two  volumes  of  absolute  al- 
cohol, and  by  further  concentration  and  precipitation  with  four  volumes 
of  absolute  alchohol,  dextrin,  etc.,  was  separated  and  estimated.  The 
mucilage  obtained  was  not  all  redissolved  in  water,  showing  the  presence 
of  some  albumen  in  this  extract,  which  was  also  estimated.  The  filtrate 
from  the  mucilage  and  dextrin  after  evaporation  of  the  alcohol  was 
precipitated  with  acetate  of  lead ;  the  precipitate,  after  weighing,  igniting 
and  deducting  the  inorganic  substances,  was  calculated  as  total  organic 
acids  and  allied  substances.  From  another  portion  of  this  filtrate  was 
estimated  quantitatively  the  glucose  from  the  cuprous  oxide  obtained 
in  an  alkaline  copper  solution  by  igniting  it  and  multiplying  by  0.45. 
Another  portion  of  the  filtrate  was  boiled  with  dilute  hydrochloric 
acid,  and  then  treated  with  Fehling's  solution ;  from  the  total  amount 
of  saccharoses  thus  estimated,  that  of  the  glucose  previously  found 
was  deducted,  leaving  the  amount  of  cane  sugar  present. 
The  powder  was  further  treated  in  succession  with  caustic  soda  (0.2 
per  cent.)  for  determining  albuminoids  and  extractive ;  with  hydro- 
