^'"sSt!i888*''°''}    Orindelia  Rohusta  and  Grindelia  Squarrosa.  435 
ANALYSIS. 
For  the  quantitative  analysis,  100  grams  of  each  drug,  in  No.  80 
powder,  were  used  and  subjected  to  the  same  treatment.  The  results 
were  similar,  hence  the  drugs  will  be  considered  together  in  the  report. 
When  not  otherwise  specified,  the  statements  made  apply  to  both. 
This  is  done  to  avoid  repetition. 
I.  Treatment  with  petroleum  ether. 
The  drug  was  exhausted  with  petroleum  ether  (boiling  point  below 
45°)  in  successive  portions.  The  amount  soluble  in  this  menstruum  is 
G.  robusta — 8.87  per  cent.  :  G.  squarrosa — 5.94  per  cent.  This  residue 
was  found  to  consist  of  vegetable  wax  and  fixed  and  volatile  oils  in  the 
following  proportions : 
G.  robusta,  wax,  0*41  per  cent.;  fixed  oil,  8*27  per  cent.;  vol.  oil,  0*19  per  cent 
G.  squarrosa,  "    0-36      "  "      5*42      "  "  0*16 
The  wax  was  of  a  white  color,  solid  at  ordinary  temperatures, 
melted  at  about  53°  C. ;  did  not  saponify  with  an  aqueous  solution  of 
soda,  but  did  with  an  alcoholic  solution  of  soda.  The  addition  of 
barium  chloride  formed  a  barium  soap,  insoluble  in  ether.  The 
alcoholic  filtrate  from  the  soap  was  treated  with  ether  and  the  ethereal 
solution  evaporated,  leaving  a  solid,  white  residue,  melting  at  about 
50°  C.,  pointing  to  cetyl  as  a  base. 
The  fixed  oil  was  solid  at  ordinary  temperatures,  melting  at  37°  C., 
and  was  of  a  brown  color.  It  gave  a  brown  color  on  the  addition  of 
sulphuric  acid.  The  oil  was  treated  with  a  solution  of  caustic  soda, 
sp.  gr.  1-26.  No  saponification  occurred,  even  on  boiling.  On  dilut- 
ing largely  with  water,  however,  it  saponified  readily,  giving  off  the 
strong  odor  of  the  drug.  Salt  was  added  in  excess  and  the  soap  col- 
lected. The  residue  was  filtered,  evaporated  and  tested  by  the  flame 
test  with  the  borax  bead  for  glycerin,  but  with  negative  results.  The 
soap  was  decomposed  with  hydrochloric  acid  and  distilled  with  water. 
The  distillate  had  oil  globules  floating  on  its  surface,  demonstrating 
the  presence  of  a  volatile  fat-acid.  Its  odor  was  aromatic  and  somewhat 
valerian-like.  The  yield  from  the  amount  examined  was  too  small 
to  determine  its  composition  by  ultimate  analysis. 
The  non- volatile  fat-acids  were  separated  by  fractional  precipitation 
with  acetate  of  magnesium  and  found  to  consist  of  a  mixture  of 
palmitic,  stearic  and  oleic  acids. 
