3io 
Phylacogens. 
{Ami.  Jour.  Pharm. 
July,  1913. 
Culture  and  Safety  Tests. 
Aerobic  and  anaerobic  culture  tests  are  made  of  each  lot  of 
Phylacogen  prepared,  to  determine  whether  the  completed  product 
is  sterile.  Coincidental  safety  tests  of  the  same  preparations  are 
made  by  injecting  relatively  large  doses  subcutaneously  into  each 
of  a  series  of  animals;  if  the  animals  remain  healthy  the  product 
is  passed.  A  large  number  of  the  test  animals  are  anesthetized, 
killed,  and  examined,  ten  days  after  injection;  in  each  instance  the 
autopsy  discloses  nothing  more  than  a  faint  trace  of  tissue  irrita- 
tion at  the  site  of  injection. 
Laboratory  Experiments. 
Careful  investigations  were  conducted  in  the  scientific  labora- 
tories for  the  purpose  of  determining  the  physiologic  effects  of  the 
Phylacogens,  and  to  demonstrate  their  safety  when  used  thera- 
peutically. These  researches  are  still  going  on,  now  more  than 
two  years  since  the  first  investigations  were  begun. 
Potency. 
The  degree  of  potency  or  energy  of  the  Phylacogens  has  been 
carefully  ascertained  by  means  of  experiments  on  laboratory  animals 
(some  eight  hundred  of  which  were  used  in  these  investigations). 
The  Phylacogens  were  injected  subcutaneously,  intravenously,  and 
intramuscularly,  and  were  given  internally.  The  results  indicate 
that  the  average  minimum  lethal  dose  (by  intravenous  injection) 
per  kilo  of  body  weight  of  an  animal  is  11.90  c.c.  By  comparison, 
it  would,  therefore,  appear  that  the  average  minimum  lethal  dose 
for  a  man  of  150  pounds  body  weight  is  about  809.2  c.c.  The 
suggested  subcutaneous  therapeutic  dose  is  2  c.c,  to  20  c.c,  for  the 
average  human  (150  lbs.  weight  or  70  kilogrammes)  or,  0.03  c.c, 
to  0.3  c.c,  per  kilo.  The  suggested  intravenous  therapeutic  dose 
is  y2  c.c  to  5  c.c,  for  the  average  human  (see  above)  or,  0.00715 
c.c  to  0.715  c.c  per  kilo.  The  relatively  (comparatively)  non- 
toxic action  of  these  Phylacogens,  therefore,  seems  assured. 
It  would  appear  from  correspondence  that  there  is  some  con- 
fusion as  to  the  potency  of  Phylacogens.  The  statement  has  been 
made  several  times  that  physicians  are  "  afraid  to  use  Phylacogens 
because  they  are  dangerous  "  and  Parke  Davis  &  Co.  have  been  re- 
