A  OctJoS/i9air9m" }    Study  °f  Strophantus  Kombe  Seeds.  683 
methods  of  M.L.D.  determinations  were  adopted.  The  following 
are  the  final  conclusions  drawn  from  59  experiments  : 
M.L.D.  by  intralymphatic  injection  for  each  kilogram  of  body 
weight  of  frog : 
0.00104  Gm.  of  K.  Strophanthin  isolated  in  the  laboratory. 
0.00107  Gm.  of  Strophanthin  Merck. 
1  Cc.  of  Tincture  of  Strophanthin  B.P. 
Oral  M.L.D.  for  each  kilogram  of  body  weight  of  frog: 
0.02  Gm.  of  K.  Strophanthin  isolated  in  the  laboratory. 
0.0208  Gm.  of  Strophanthin  Merck. 
20.40  Cc.  of  Tincture  of  Strophanthus  B.P. 
From  these  results  one  may  conclude  that : 
(1)  For  a  frog  the  oral  M.L.D.  is  about  twenty  times  more  than 
the  M.L.D.  given  by  intralymphatic  injection. 
(2)  The  toxicity  of  K.  Strophanthin  (isolated  in  the  laboratory) 
is  practically  identical  with  that  of  Strophanthin  Merck. 
Part  III. 
The  same  B.P.  Tincture  of  Strophanthus  whose  M.L.D.  was 
determined  in  Part  II.  was  assayed  by  the  following  methods  and 
gave  the  following  results  : 
(i)  0.082  per  cent,  of  Strophanthin  in  the  tincture  as  assayed  by 
Elborne's  method  ("Year-Book  of  Pharmacy,"  1887,  423). 
(ii)  0.086  per  cent,  of  Strophanthin  in  the  tincture  as  assayed  by 
Fraser's  method  (P. J.,  1889,  332). 
(iii)  0.097  per  cent,  of  Strophanthin  in  the  tincture  as  assayed  by 
Barclay's  method  (P. J.,  Nov.  28,  1896,  463). 
(iv)  0.102  per  cent,  of  Strophanthin  in  the  tincture  as  assayed  by 
Fromme's  1910  method  (E.  Ph.,  vol.  II.,  1915,  p.  129). 
(v)  0.101  per  cent,  of  Strophanthin  in  the  tincture  as  assayed  by 
Lampart  and  Mueller's  method  (A.  Pharm.,  ccli.,  609). 
(vi)  0.104  per  cent,  of  Strophanthin  in  the  tincture  physiologically 
assayed. 
The  above  results  show  that  Barclay's,  Fromme's  1910,  and  Lam- 
part and  Mueller's  methods  agree — within  limits — with  the  physio- 
logical standardization  and  are,  therefore,  satisfactory. 
It  was  noted  that  it  would  be  preferable  to  use  a  perforator — - 
instead  of  shaking  out  in  a  separating  funnel — in  removing  the 
strophanthidin  by  chloroform,  as  this  would  minimize  the  amount  of 
