Am'  Sept"'  1^19™"  }    Standardization  of  Piscidia  erythrina. 
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ize  himself  with  the  behavior,  peculiarities,  etc.,  of  the  dog  under 
normal  conditions.  The  same  animal  may  be  used  many  times, 
provided  that  twenty-four  to  thirty-six  hours  are  allowed  to  elapse 
between  doses  in  order  that  the  animal  may  completely  recover  from 
the  effects  of  the  previous  dose. 
Although  the  animals  never  appear  to  lose  their  susceptibility,  it 
is  not  advisable  to  use  a  dog  for  more  than  six  months,  and  care 
should  be  taken  to  allow  one  week  to  elapse  between  assays. 
Preparation  of  Experiment. — Select  and  weigh  several  animals 
which  have  been  found  easily  susceptible  to  the  action  of  Jamaica 
dogwood,  and  withhold  all  food  for  at  least  twelve  hours  previous 
to  the  time  of  administration  of  the  drug.   Water  should  be  allowed. 
Fig.  2.  Same  dog  as  shown  in  Fig.  i,  one  hour  after  receiving  a  dose  of 
active  Jamaica  dogwood.  This  figure  clearly  illustrates  the  stage  of  inco- 
ordination produced  by  Jamaica  dogwood.  That  the  dog  has  lost  control  of 
the  hind  legs  and  of  the  muscles  supporting  the  head  can  be  noted  by  the 
drooping  of  the  head  and  hind  quarters.  Also  note  that  the  legs  are  spread 
apart  in  order  to  maintain  balance. 
Preparation  of  Drug  for  Administration. — Tinctures,  solid, 
powdered,  and  fluid  extracts,  are  weighed  or  measured  directly  into 
hard  gelatin  capsules.  When  a  crude  drug  is  to  be  tested  a  rep- 
resentative sample  should  be  finely  ground  and  then  made  into  a 
fluid  extract. 
Method  of  Administering. — The  drug  is  administered  internally 
by  means  of  a  small  capsule.    The  animal's  mouth  is  opened  by 
