302 
The  Tannin  of  Cloves. 
/  Am.  Jonr.  Pliarru. 
\       Jane.  1895. 
coloring  matter,  but  no  tannin.  The  aqueous  layer  from  the  above 
operation  was  saturated  with  sodium  chloride  and  again  shaken  with 
acetic  ether.  In  the  presence  of  the  sodium  chloride,  the  coloring 
matter  and  almost  all  of  the  tannin  were  readily  removed  from  the 
aqueous  liquid  by  agitation  with  three  successive  portions  of  acetic 
ether.  These  were  united  and  the  solvent  recovered.  The  residue 
so  obtained  was  treated  with  water,  which  left  considerable  wax,  oil 
and  resin  undissolved.  The  solution  was  filtered  and  the  filtrate 
shaken  with  acetic  ether  as  before.  The  acetic  ether  layer  was  sep- 
arated and  the  solvent  recovered.  The  residue  left  was  treated  with 
water  and  the  filtered  solution  shaken  with  acetic  ether  in  the  man- 
ner described.  After  several  repetitions,  this  process  indicated  its 
value  to  separate  the  resinous  constituents  from  the  tannin.  But  a 
considerable  waste  of  that  principle  was  found  to  have  occurred 
when  the  acetone  extract  was  treated  with  water  for  the  first  time. 
To  obviate  this  loss,  which  arose  through  precipitation  of  the  tan- 
nin along  with  the  resin,  a  second  method  of  purification  was  insti- 
tuted. This  consisted  in  mixing  the  acetone  percolate  of  another 
lot  of  the  same  quality  of  cloves,  and  from  which  the  solvent  had 
not  been  recovered,  with  sufficient  water  to  completely  precipitate  it. 
Paper-pulp  was  uniformly  distributed  throughout  the  unfiltered  mix- 
ture in  order  to  fulh'  clarify  the  aqueous  solution.  This  treatment 
proved  successful ;  the  pulp  retained  the  oil,  wax  and  resin  so  effec- 
tually that  a  clear  liquid  was  obtained  by  simple  filtration.  Some 
acetone  was  added  to  this  liquid  to  replace  that  lost  by  evaporation, 
and  the  entire  solution  afterwards  saturated  with  sodium  chloride. 
The  last  substance  caused  the  acetone  to  separate  as  a  supernatant 
layer.  When  this  was  removed  and  the  solvent  recovered,  a  con- 
siderable quantity  of  tannin  was  obtained.  Further  agitation  with 
two  successive  portions  of  acetone  sufficed  to  exhaust  the  aqueous 
layer  of  tannin. 
The  product  obtained  upon  the  recovery  of  the  acetone  was  equal 
in  purity  to  that  in  hand  when  the  process  of  repetition  at  first 
employed  was  discontinued,  while  the  attendant  waste  of  tannin 
was  very  much  less. 
From  both  the  first  and  second  methods  of  isolation,  the  tannin, 
upon  the  recovery  of  acetic  ether  or  acetone,  was  obtained  in  a 
porous  or  "  puffed-up  "  condition.  The  products  of  both  processes 
were  dissolved  in  the  same  portion  of  water,  the  solution  treated 
