Am.  Jour.  Pharru. 
June,  1895. 
Anatolian  Licorice  Root. 
307 
tillation,  after  which  operation,  to  estimate  their  amounts,  the 
entire  extracts  were  transferred  to  tared  beakers  and  evaporated  to 
constant  weights  on  a  water-bath. 
The  total  solids  of  the  water,  alkaline  water  and  acidulated  water 
extracts  were  determined  by  evaporating  an  aliquot  part  of  the 
solution  to  a  constant  weight  by  the  means  already  described.  The 
residue  was  ignited  and  the  resulting  ash  deducted  from  the  weight 
of  total  solids.    The  difference  was  taken  as  organic  solids. 
Both  in  the  absolute  alcohol  and  water  extracts,  glucose  was 
tested  for  and  estimated  by  making  the  liquid  alkaline  with  sodium 
hydrate  and  then  heating  with  Fehling's  solution  for  twenty  minutes 
on  a  water-bath.  The  cuprous  oxide  was  then  collected,  washed, 
dried  and  converted  by  ignition  into  cupric  oxide.  Forty-five  per 
cent,  of  the  weight  of  the  latter  was  considered  to  equal  the  amount 
of  glucose  present  in  the  fractional  part  of  the  extract  under  exam- 
ination. To  estimate  the  sucrose  an  equal  volume  of  the  glucose 
containing  liquid  was  boiled  for  one-half  hour  with  a  small  quantity 
of  hydrochloric  acid  to  convert  the  former  into  the  latter  sugar. 
After  this  treatment,  the  liquid  was  allowed  to  cool,  and,  after  having 
been  made  alkaline,  was  treated  with  Fehling's  solution,  as  in  the 
case  of  glucose  alone.  The  increase  in  the  amount  of  cupric  oxide 
obtained  upon  the  ignition  of  the  cuprous  oxide,  that  resulted  irom 
this  treatment,  was  attributed  to  sucrose,  the  amount  of  which  was 
calculated  as  95  percent,  of  the  amount  of  glucose  represented  by 
the  additional  cupric  oxide. 
To  obviate  any  discrepancy  that  might  arise  from  the  presence  of 
extractive  or  glycyrrhizin,  the  water  solution,  previous  to  the  appli- 
cation of  the  process  for  the  determination  of  glucose  and  sucrose, 
was  completely  precipitated  with  neutral  lead  acetate.  After  filter- 
ing the  mixture,  the  excess  of  lead  was  thrown  out  with  hydrogen 
sulphide.  The  resulting  lead  sulphide  was  separated  by  filtration. 
The  filtrate  was  warmed  to  expel  hydrogen  sulphide,  then  allowed 
to  cool,  and  afterwards  made  up  to  a  definite  volume.  Portions  of 
this  were  then  used  for  the  determinations  of  the  sugars. 
The  percentages  of  constituents  stated  in  this  paper  are  adjusted 
on  the  air-dry  drug. 
Petroleum  Ether  Extract. — Petroleum  ether  extracted -54  per  cent, 
of  the  weight  of  the  root.  Only  a  slight  loss  in  weight  occurred 
when  this  extract  was  heated  to  1200  C.    Treatment  with  hot  alco- 
