^ctSbS,^150'}    Acidimetric  Estimation  of  Alkaloids.  505 
10  per  cent,  ammonia  water,  then  agitate  frequently  and  during 
one  hour.  On  adding  5  grammes  more  of  10  per  cent,  ammonia 
water  and  shaking  well,  the  suspended  powder  agglutinates  into  a 
lump,  the  liquid  becomes  clear  after  standing  a  few  minutes  and  can 
be  poured  off  almost  completely. 
A.  When  the  mixture  has  completely  separated,  pour  off  50 
grammes  into  a  beaker,  evaporate  the  solvent  on  a  water  bath, 
add  10  c  c.  of  ether  and  evaporate  again.  Dissolve  the  varnish-like 
residue  in  15  c.c.  of  alcohol,  with  heat,  add  water  to  slight  perma- 
nent turbidity,  the  requisite  quantity  of  indicator  and  an  excess  oi 
the  acid  solution;  retitrate  with  the  centinormal  alkaline  solution. 
La  Wall. 
Kebler. 
La  Wall. 
Kebler. 
La  Wall. 
Kebler. 
La  Wall. 
Kebler. 
Indicators. 
Per  Cent,  of  K\r 
kaloids  in  Nux 
Vomica  by  Pro- 
cess A . 
Per  Cent,  of  Al- 
kaloids in  Nux 
Vomica  by  Pro- 
cess B,  Gravi- 
metrically. 
Per  Cent,  of  Al- 
kaloids in  Nux 
Vomica  by  Pro- 
cess  B,  Volu- 
metrically. 
Per  Cent,  of  Al- 
kaloid in  Ipe- 
cac   Root  by 
Process  A. 
B.  W  
C  
H  
%  
2-04 
2-64 
2'l8 
2-  38 
3-  02 
2-58 
2*69 
2-24 
2-34 
3  64 
2-94 
2-86 
2-88 
2'93 
2  "93 
3'oo 
3-10 
3-11 
3*05 
3-02 
2-37 
2*42 
2-23 
2"55 
265 
2*37 
2'39 
2-27 
2-37 
2-61 
2-46 
2*59 
2*48 
2'55 
2*95 
2-54 
2-49 
254 
2-57 
3*30 
Per  Cent,  of  Al- 
kaloid in  Ipe- 
cic    Root  by 
Process  B, 
Gravim  e  t  r  i  c- 
ally. 
Per  Cent,  of  Al- 
kaloid in  Ipe- 
cac   Root  by 
Process  B.  Vol- 
umetrically. 
Per  Cent,  of  Al- 
kaloids in  Bel- 
ladonna Leaves 
by  Process  B, 
Gravimetric- 
ally. 
Per  Cent,  of  Al- 
kaloids in  Bel- 
ladonna Leaves 
by  Process  B, 
Volumetrically, 
B.  W  
H  
I,  
M.  O  
258 
2-63 
2-58 
2*62 
2-66 
2  60 
2*68 
2-68 
2  "60 
2-63 
2-36 
2*52 
2'35 
2*40 
2-89 
2*35 
2'33 
2'33 
2-25 
261 
0*26 
0-28 
0*27 
0*24 
0-25 
0*20 
0'20 
0'22 
0-18 
0*20 
0*19 
0*24 
0"2I 
0"20 
0-23 
0-15 
o'i4 
0-13 
o'i5 
0'20 
Note.— B.  W.  =  Brazil  Wood,  C.  =  Cochineal,  H.  =  Hsematoxylin,  L.  =  Litmus,  and 
M.  O.  =  Methyl  Orange. 
B.  When  the  mixture  has  completely  separated,  pour  50  grammes 
into  a  separatory  funnel,  treat  at  once  with  20  c.c.  of  acidulated 
water;  after  thorough  agitation  and  complete  separation,  remove 
the  aqueous  solution  into  a  second  separatory  funnel.  Repeat  the 
above  operation  twice  more  successively  with  15  c.c.  of  slightly 
acidulated  water.  The  acidulated  water  in  the  second  separatory 
funnel  is  rendered  alkaline  with  ammonia  water,  the  alkaloid 
removed,  successively,  with  20  c.c,  15  c.c,  and  15  c.c,  of  a  mixture 
of  three  parts  (by  volume)  of  chloroform  and  one  part  of  ether. 
