506  Acidimetric  Estimation  of  Alkaloids.    {A  oct?ber,Pi895[m' 
Collect  the  chloroform- ether  mixture  in  a  tared  beaker,  and  distill 
off  the  solvent.  The  varnish-like  residue  is  twice  treated  with  8  c.c. 
of  ether,  evaporated  on  a  water  bath  and  dried  to  constant  weight 
on  the  water  bath.  The  varnish-like  residue  is  dissolved  in  1 5  c.c. 
of  alcohol  and  treated  as  in  A  above. 
Nux  vomica  and  ipecac  root  were  treated  according  to  processes 
A  and  B  ;  belladonna  leaves  according  to  process  B. 
According  to  the  well-established  method  of  Messrs.  Dunstana 
and  Short,  the  nux  vomica  examined  contained  2*89  per  cent,  of 
crude  alkaloid.  On  carefully  titrating  this  crude  product  with  a 
volumetric  acid  solution,  2-12  per  cent,  of  pure  alkaloid  was  indi- 
Indicators. 
Per  Cent,  of  Alka- 
loids in  Fluid  Ex- 
tract of  Nux  Vom- 
ica by  Process  A. 
Per  Cent,  of  Alka- 
loids in  Fluid  Ex- 
tract of  Nux  Vom- 
ica by  Process  B, 
Gravimetrically. 
Per  Cent,  of  Alka- 
loids in  Fluid  Ex- 
tract of  Nux  Vom- 
ica by  Process  B, 
Volumetrically. 
Ea  Wall. 
Kebler. 
Ea  Wall. 
Kebler. 
Ea  Wall. 
Kebler. 
B.  W  
C  
H  
E  
M.  O  
1-  62 
177 
r6t 
1*69 
2-  OI 
170 
176 
1-67 
172 
1*92 
202 
1-97 
2"0I 
2'02 
2'0I 
2'00 
2'0I 
1*96 
1-94 
1 '94 
170 
174 
1-58 
1 '59 
1-92 
r6i 
1-64 
1-50 
1 '55 
1  82 
Per  Cent,  of  Alka- 
loid in  Fluid  Ex- 
tract of  Ipecac 
Root  by  Process 
A. 
Per  Cent,  of  Alka- 
loid in  Fluid  Ex- 
tract of  Ipecac 
Root  by  Process  B, 
Gravimetrically. 
Per  Cent,  of  Alka- 
loid in  Fluid  Ex- 
tract of  Ipecac 
Root  by  Process  B, 
Volumetrically. 
B.  W  
H  
1/  
174 
179 
1  77 
174 
200 
178 
i-8o 
r86 
174 
1  "95 
175 
176 
174 
173 
174 
r86 
1  80 
i'74 
i;8o 
179 
1-67 
172 
i'65 
r66 
1*92 
1-67 
172 
1-65 
i"6o 
i-88 
cated.  Cochineal  was  used  as  indicator.  These  figures  show  that 
this  method  produces  an  alkaloidal  residue  containing  a  smaller 
percentage  of  pure  alkaloid  than  that  obtained  by  Keller's  process. 
The  writer's  attention  was  next  directed  to  the  fluid  extracts  of 
nux  vomica  and  ipecac  root,  whose  alkaloids  were  extracted  as 
follows:  place  12  grammes  of  the  extract  into  a  250  c.c.  flask,  dilute 
with  12  grammes  of  distilled  water,  agitate  well,  then  add  30 
grammes  of  chloroform  and  90  grammes  of  ether,  stopper  the  flask 
securely  and  shake  well.  Add  6  grammes  of  10  per  cent,  ammonia 
water,  and  agitate  the  mixture  frequently  during  half  an  hour. 
a  1883,  Pharm.  J.  Trans.  (3),  13,  665. 
