148  Chemical  Examination  of  Cincho-^inine.{^'^-^^^l ^^l'"^- 
color  to  moist  red  litmus  paper,  and  produced  copious  fumes  when  a 
glass  rod,  moistened  with  hydrochloric  acid,  was  subjected  to  its  influ- 
ence. Upon  evaporating  a  portion  of  the  solution,  a  crystalline  residue 
was  obtained,  which,  heated  strongly  in  a  platinum  capsule,  was  com- 
pletely volatilized. 
Qualitative  result  No.  3. — Cincho-quinine  contains  sulphate  of  ammonium. 
B. — The  hot  alcoholic  solution  deposited  on  cooling  an  abundance  of 
crystals,  which  were  collected  on  a  filter.  The  crystals==5  2.  The 
filtrate— 5  i. 
B  I. — A  portion  of  the  cold  filtrate  was  diluted  with  water,  acidula- 
ted with  hydrochloric  acid,  and  treated  with  chloride  of  barium,  which 
formed  an  abundant  precipitate  of  sulphate  of  barium.  The  remainder 
of  the  solution  was  evaporated  to  dryness  with  gentle  heat.  During  the 
evaporation  a  white,  apparently  crystalline  ring  was  formed  on  the  sides 
of  the  capsule  and  near  the  original  level  of  the  solution.  As  the  evap- 
oration proceeded  a  second  resinous  ring  formed  further  down  the  sides 
of  the  capsule.  Finally,  when  the  evaporation  was  completed,  a  crys- 
talline deposit  formed  in  the  centre  of  the  capsule.  Portions  of  each 
of  the  ring  deposits  were  tested  with  chlorine  water  and  ammonia, 
but  gave  no  indication  of  either  quinia  or  quinidia.  The  entire  residue 
was  then  dissolved  in  acidulated  water,  the  solution  precipitated  with 
ammonia,  then  shaken  with  ether,  and  the  ethereal  solution  evaporated. 
The  small  residue  so  obtained  was  dissolved  in  dilute  acid,  and  gave, 
when  treated  with  chlorine  water  and  ammonia,  a  green  color  ;  with 
chlorine  water,  ferrocyanide  of  potassium  and  ammonia,  a  red  color. 
Qualitative  result  No.  4. — Cincho-quinine  contains  either  quinia or  qui- 
nidia.^ or  both. 
B  2. — The  crystals,  deposited  on  the  cooling  of  the  hot  alcoholic  solu- 
tion (5),  were  digested  with  water,  transferred  to  a  filter,  and  washed 
with  water  several  times.    The  solution=^7.     The  residual  crystals=Z'. 
a.  The  solution  was  found  to  contain  sulphuric  acid  in  abundance. 
It  afforded  a  copious  precipitate  on  the  addition  of  ammonia.  No  color 
reaction  was  produced  by  either  chlorine  water  and  ammonia,  or  chlorine 
water,  ferrocyanide  of  potassium  and  ammonia. 
Qualitative  result  No.  5. — Cincho-quinine  contains  sulphate  of  cinchonia. 
h.  The  crystals,  which  remained  undissolved  by  digestion  with  water 
{B  2),  were  readily  dissolved  by  dilute  hydrochloric  acid.  The  absence 
of  sulphuric  acid  was  proved  by  the  usual  test.  The  absence  of  quinia 
or  quinidia  was  also  proved  in  the  same  manner  as  in  experiment  a. 
