AmM°ayr*i92iarm'}     Scientific  and  Technical  Abstracts. 
361 
tional  distillation  of  ethyl  esters  under  high  pressure,  though  consid- 
ered promising,  was  abandoned  in  favor  of  (4)  direct  fractional 
distillation. 
By  the  last  method  four  esters  of  the  fatty  acids  of  the  oil  were 
developed  and  were  used  on  four  groups  of  patients,  a  considerable 
number  of  whom  become  clinically  and  bacteriologically  free  from 
leprosy.  But  it  was  impossible  to  identify  this  effect  with  anyone  of 
the  esters. 
Determination  of  Lactose  in  Milk  by  Colorimetric 
Method. — The  method  is  a  simple  adaption  of  Folin's  latest  blood 
sugar  determination  to  the  estimation  of  lactose. 
Method :  To  1  c.  c.  of  milk  in  a  100  c.  c.  flask  add  2  c.  c.  of  10  per 
cent,  sodium  tungstate  solution  and  2  c.  c.  two-thirds  normal  sul- 
phuric acid,  the  latter  drop  by  drop.  Mix  and  let  stand  five  min- 
utes ;  dilute  to  100  c.  c.  and  filter.  Place  1  c.  c.  of  filtrate  and  1  c.  c. 
of  water  in  a  Folin  sugar  tube ;  to  the  second  sugar  tube  add  2  c.  c. 
of  standard  lactose  solution  A.  And  to  a  third  tube  add  2  c.  c.  of 
standard  lactose  solution  B.  To  each  of  the  three;  tubes  add  2  c.  c. 
of  alkaline  copper  solution  and  boil  for  six  minutes.  Cool  in  water, 
add  2  c.  c.  of  Folin  molybdate  phosphate  solution  and  let  stand  a  few 
minutes.  Standard  A  contains  0.5  mg.  lactose  per  2  c.  c.  Standard 
B  contains  0.7  mg.  per  2  c.  c.  The  reading  of  the  standard  divided 
by  reading  of  unknown,  multiplied  by  5  if  Standard  I  is  used,  or  7  if 
Standard  II  is ;  used  gives  the  grams  of  lactose  per  100  c.  c.  of  milk. 
To  make  the  Standard  lactose  solution  make  an  accurate  1  per 
cent,  lactose  solution  in  distilled  water.  Preserve  with  toluol.  For 
Standard  I  dilute  12.5  c.  c.  of  the  stock  solution  to  500  c.  c.  For 
Standard  II  dilute  17.5  c.  c.  of  stock  solution  to  500  c.  c. — R.  G. 
Owen  and  Roth  Gregg,  /.  Lab.  &  Clin.  Med.,  Jan.,  192 1. 
