794 
Modified  Test  for  Glucose. 
(  Am.  Jour.  Pharm. 
I       Nov.,  1921.  ' 
for  four  weeks.  Avoid  salt  in  diet,  as  it  increases  absorption  of  the 
mercury. 
14.  Give  by  continuous  proteolysis  a  solution  containing  1  dram 
of  potassium  acetate,  4  drams  of  glucose,  and  3  drams  of  sodium 
carbonate  to  the  pint. 
15.  Keep  the  urine  alkaline  to  methyl  red. 
16.  Continue  rest  treatment  until  recovery,  usually  a  period 
of  three  weeks. 
HAINES  MODIFIED  TEST  FOR  GLUCOSE.* 
As  far  back  as  1874,  W.  S.  Haines  1  introduced  a  test  for  sugar 
in  the  urine  which  was  regarded  as  more  delicate  than  the  methods 
in  common  use.  Recently  the  same  investigator,  together  with  G. 
P.  Pond  and  R.  W.  Webster,2  published  an  improved  test  capable 
of  detecting  with  certainty  amounts  about  0.03  per  cent,  of  sugar, 
which  is  about  the  upper  limit  of  the  so-called  "normal"  sugar  of 
the  urine.  That  is,  pathological  sugar  will  be  indicated,  but  physio- 
logical sugar  will  go  undetected. 
The  composition  of  the  improved  Haines  solution  is : 
Copper  sulphate   5  gm. 
Glycerin   ."   250  cc. 
Potassium  hydroxide    20  gm. 
Distilled  water  to    1000  cc. 
The  copper  sulphate  is  dissolved  in  a  mixture  of  the  glycerin 
and  an  equal  amount  of  water,  with  the  aid  of  gentle  heat.  The 
potassium  hydroxide  should  be  dissolved  in  about  200  cc.  of  water 
and  added  to  the  copper  solution  with  constant  stirring,  the  whole 
being  made  up  to  volume  with  distilled  water.  This  solution  keeps 
indefinitely,  although  with  many  of  the  specimens  of  glycerin  now 
obtainable  on  the  market  a  reduction  may  be  observed.  If,  however, 
the  solution  be  allowed  to  stand  in  a  warm  place  for  forty-eight 
hours,  the  clear  supernatant  fluid  may  be  decanted  or  filtered  from 
the  precipitated  cuprous  oxide,  without  impairing  its  delicacy. 
*From  The  Prescriber,  1921,  p.  291. 
1  Med.  Examiner,  Dec.  1,  1874,  P-  569. 
2  J.  Amer.  Med.  Assoc.,  Jan.  31,  1920,  p.  301. 
