464 
Glycyrrhizin  in  Licorice  Root. 
5  Am.  Jour.  Pharm. 
I      July,  1921. 
95  per  cent,  alcohol  are  then  added  with  stirring,  and  then  more 
alcohol  up  to  the  301  mark.  After  thorough  shaking,  the  contents 
of  the  flask  are  transferred  to  a  conical  flask,  which  is  stoppered 
up,  and  the  gums,  etc.  are  allowed  to  settle  for  2  hours ;  the  clear 
liquid  is  then  poured  off  into  a  vessel  graduated  at  150  cc.  When  the 
precipitate  begins  to  pass  over,  it  is  filtered  through  a  fluted  filter  of 
19  cm.  diameter,  until  150  cc.  filtrate  is  obtained.  The  filtrate  is  trans- 
ferred to  an  evaporating  dish  and  evaporated  to  25  cc. 
'The  further  procedure  is  the  same  as  for  the  first  method." 
[Translator's  Note. — Linz  points  out  that  this  method  is  in- 
tended for  rapid  analysis,  and  saves  24  hours  standing.  Linz  con- 
cludes that  this  more  rapid  method  gives  results,  which  hardly  differ 
from  Gadais  1.  (P.  A.  H.)] 
15.  Trabeck  (ipoo). 
"Two  g.  licorice  are  dissolved  in  5  cc.  water,  starch  and  gums 
precipitated  with  20  cc.  96  per  cent,  alcohol,  and  the  residue  filtered 
and  washed  with  dilute  alcohol  (4  alcohol!  to  1  water)  until  the  fil- 
trate is  colorless.  The  filtrate  is  evaporated  to  about  1.5  cc,  the  residue 
is  dissolved  in  2  cc.  glacial  acetic  acid,  and  30  cc.  absolute  alcohol  ' 
are  added  with  shaking.  After  standing  to  settle  the  precipitate, 
filter  through  a. tared  filter,  wash  with  absolute  alcohol  until  neutral, 
dry  three  hours  at  a  1050,  and  weigh.  The  precipitate  does  not 
consist  of  glycyrrhizin  only,  but  also  contains  alkalis." 
[Translator's  Note.— Linz  points  out  that  glycyrrhizin  com- 
pounds are  probably  precipitated  with  starch  and  gums,  by  the  use 
of  so. much  strong  alcohol.  Linz  also  points  out  a  number  of  other 
objections,  which  render  this  method  without  value,  and  he  also  states 
that  it  is  not  possible  to. improve  it,  with  a  view  to  making  it  workable. 
(P.  A.  H.)] 
16.  Schroder  (1884). 
[Translator's  Note. — This  method  uses  repeated  precipitation 
with  sulphuric  acid,  but  gives  absolutely  no  details  as  to  quantities. 
Linz  points  out  that  the  losses  from  repeated  precipitations  are 
obviously  very  great,  and  that  the  method  has  only  historical  interest. 
(P.  A.  H.)] 
