466  Glycyrrhizin  in  Licorice  Root.  \Amjliy*\marm' 
the  acid  weighed,  very  impure.  It  gives  a  strong  chlorine  reaction 
and  consequently,  give  results  which  are  too  high.  Linz  rejects  this 
method.  (P.  A.  H.)] 
20.  Kinzey  (1898). 
A  mixture  of  40  cc.  of  ammonia,  240  cc.  of  alchhol,  and  water 
up  to  1000  cc.  is  used  as  an  extraction  liquid. 
[Translator's  Note. — Linz  justly  condemns  this  method  on 
several  grounds.  The  glycyrrhizin  is  precipitated  from  much  too 
large  a  volume,  and  it  is  precipiated  by  sulphuric  acid  in  the  presence 
of  alcohol,  whereas  the  alcohol  should  obviously  be  removed.  The 
use  of  dilute  acetic  acid  to  wash  the  precipitated  glycyrrhizin,  results 
in  further  loss,  and  the  quantity  of  licorice  used  results  in  much  too 
small  a  quantity  of  glycyrrhizin  being  weigned  for  an  accurate 
method. 
Linz  concludes  that  Kinzey's  method  is  without  value. 
(P.  A.  H.)] 
21.  Anselmino-Gilg  (ion). 
[Translator's  Note. — This  method  is  adapted  from  that  of 
Kremel,  some  of  whose  missing  instructions  are  here  supplied.  The 
original  solution  is  made  with  ammoniacal  water.  Linz  points  out 
that  a  considerable  loss  of  glycyrrhizin  results  from  washing  with 
50  cc.  water.  Linz  further  states  "for  fundamental  reasons  stated  in 
the  introduction  I  object  to  the  use  of  an  ammoniacal  extract.  From 
the  large  quantity  of  water  used  in  washing,  considerable  errors 
result.    There  are  no  other  objections  to  this  method."  (P.  A.  H.)] 
22.  Stoeder  (/pox). 
[Translator's  Note. — This  method  also  uses  ammoniacal 
water  to  dissolve  the  original  licorice  extract.  Linz  points  out  that 
the  quantity  of  wash-water  prescribed  is  far  short  of  that  necessary, 
resulting  in  ammonium  chloride  being  mixed  in  with  the  glycyr- 
rhizin weighed.  The  glycyrrhizin  is  also  very  impure  on  account  of 
the  original  ammoniacal  extraction.  (P.  A.  H.)] 
23.  Telle  (ip 11). 
"2.5  g.  licorice  extract  are  dissolved  in  20  cc.  of  water  in  a 
centrifuge  tube,  and  whirled  for  fifteen  minutes.   The  clear  liquid  is 
poured  off,  the  res;due  is  mixed  with  ammoniacal  water  (10  cc.  of 
