344 
CINCHO-QUININE." 
analogous  to  one  of  sulphate  of  cinchonia,  and  the  solution  when 
strongly  acidulated  with  the  acid,  possessed,  in  very  slight  de- 
gree only,  the  optical  phenomena  of  fluorescence  and  epipolism. 
Dr.  Bill's  test  of  ferro-cyanide  of  potassium  gave  the  known 
reaction  for  cinchonia.  ^'  Cincho-Quinine  "  was  nearly  insoluble 
in  ether.  Twenty  grains  of  the  preparation  were  dissolved  in 
water  with  a  sufficient  quantity  of  sulphuric  acid,  and  the  solu- 
tion subjected  to  Liebig's  ether  test,  which  dissolves  quinia, 
quinicia  and  cinchonicia,  also  portions  of  quinidia  and  cincho- 
nidia,  if  a  large  excess  of  ether  be  employed.  The  etherial  so- 
lution thus  obtained  by  successive  washings  with  ether,  left  on 
evaporation  and  drying  a  solid  residue  weighing  about  half  a 
grain,  possessing  alkaloidal  properties.  This  residue  when  dis- 
solved in  dilute  sulphuric  acid  and  water,  and  treated  with 
Brando's  chlorine  and  ammonia  test,  will  indicate  by  its  green 
coloration  the  presence  of  quinia,  quinidia  and  quinicia.  The 
test  responded  in  this  instance  affirmatively.  In  order  to  deter- 
mine which  of  the  alkaloids  produced  the  coloration,  one  portion 
of  the  solution  was  tested  for  quinidia  by  Van  Heijningen's  test 
of  oxalate  of  ammonia,  and  another  portion  was  tested  for  quini- 
dia by  Dr.  Vry's  test  of  iodide  of  potassium,  but  both  gave  nega- 
tive results.  Therefore  the  alkaloid  detected  by  Brande's  test 
is  quinicia,  which  was  confirmed  by  the  application  of  Hera- 
path's  optical  and  chemical  tests  of  the  iodo-sulphates  of  the 
cinchona  alkaloids.  One  grain  of  the  mixed  alkaloids  obtained 
by  Liebig's  test  from  "  Cincho-Quinine  "  by  thorough  exhaustion 
with  ether,  was  dissolved  in  a  fluid  drachm  of  water  sufficiently 
acidulated  with  sulphuric  acid.  The  solution  was  then  mixed 
with  an  equal  bulk  of  alcohol,  the  mixture  warmed  to  about  100° 
Fahr.  and  treated  successively  with  tincture  of  iodine.  The 
several  (7)  precipitates  which  appeared  on  cooling  were  amor- 
phous resinous  substances  soluble  in  alcohol,  and  did  not  exhibit 
in  the  least  degree  crystalline  structures.  The  precipitates  first 
obtained  were  reddish  in  appearance,  analogous  to  the  salt  of 
iodo-sulphate  of  quinicia;  the  last  precipitates  possessed  the  pur- 
plish tint  belonging  to  the  iodo-sulphate  of  cinchonicia.  The 
absence  of  all  crystalline  characteristics  of  iodo-sulphate  salts 
thus  obtained  from  the  alkaloids  extracted  by  ether  from  "  Cin- 
