Am.  .Tour.  Pharaj. 
May,  1901. 
Animal  Parasites  in  Man. 
233 
certain  geographical  districts.  To  detect  the  ova  allow  the  urine 
to  stand  until  all  blood  clots  are  collected  at  the  bottom  of  the  tube; 
(2)  lift  a  portion  of  this  sediment  into  a  pipette  and  place  a  drop  on 
the  centre  of  a  slide;  (3)  tease  the  clots  as  fine  as  possible,  and 
evaporate  nearly  to  dryness ;  (4)  add  a  drop  of  cast  medium,  or 
glycerine,  to  the  centre  of  the  specimen  upon  which  place  a  cover- 
glass  and  spread  the  medium  by  additional  pressure.  The  specimen 
should  be  placed  on  a  flat  surface  for  twenty-four  hours  while  the 
mounting  medium  hardens,  after  which  time  a  permanent  ring  may 
be  added.  For  rapid  diagnosis  the  specimen  may  be  mounted  in 
water.  Detection  of  these  ova  is  best  accomplished  by  the  lens 
{Fig.  6).  Individual  ova  may  be  studied  under  a  higher  power,  when 
Fig.  5. — Trichina  spiralis.  Eighth  Fig.  6. — Bilharz's  parasite.  (i)Ova 
week  of  disease.  (B.  L.,  %);  (2)  ova  (B.  Iv.,  Ye). 
it  is  often  possible  to  distinguish  the  contained  embryo  which  varies 
in  its  appearance  with  the  age  of  the  egg.  Influenced  by  tempera- 
ture, these  embryos  are  freed  from  their  shell  in  from  a  few  hours 
to  several  days  after  they  are  passed  with  the  urine.  The  most 
immature  ova  are  about  inch  in  length  and  -g-J^-  inch  in 
breadth,  while  fully  matured  ovum  measures  inch  in  length 
and  -g-l-g-  inch  in  breadth.  The  study  of  ova  in  feces  needs  no 
special  explanation. 
Th£  Voi,atii,e  Oil,  OF  Buchu,  according  to  Kondakow  and  Bachtschiew, 
consists  of  (1)  a  mixture  of  limonene  and  dipentene  (10  per  cent.);  (2)  inen- 
thone  (60  per  cent.);  (3)  diosphenol,  (20  per  cent.);  (4)  resinous  matter,  (5 
per  cent.). — Ph.  Zeit.,  1901,  194.. 
