344 
Story  of  the  Pap  aw. 
Am.  Jour.  Pharm. 
July,  1901. 
acid,  and  then  saturated  with  common  salt,  gives  a  white  floccu- 
lent  precipitate  (primary  albumose).  After  saturation  with  am- 
monium  sulphate,  the  filtrate  gives  a  precipitate,  deutero-albumose, 
and  the  supernatant  liquid,  under  the  biuret  test,  shows  the  pres. 
ence  of  peptones.15  If  precipitated  by  soda-magnesium  sulphate,  the 
filtrate  likewise  exhibits  a  strong  peptone  reaction.16 
ANALYSIS  OF  PAPAW  PROTEIDS. 
It  cannot  be  said  that  any  of  the  enzymes  have  been  completely 
isolated.  The  most  that  can  be  urged  is  that  the  enzymes  are 
either  proteid  in  character,  or  are  associated  with  proteid  bodies. 
In  all,  or  nearly  all,  attempts  to  separate  the  enzyme  from  the 
accompanying  proteid,  the  result  has  been  a  destruction  of  enzymic 
power.  Again,  when  in  our  manipulation  of  the  enzymes  we  alter 
or  destroy  the  character  of  the  proteids  which  are  associated  with 
them,  we  alter  or  destroy  the  character  of  the  enzyme.  While  it 
cannot  be  said  that  the  enzyme  and  the  proteid  are  identical,  we 
must  admit  that  the  enzyme  and  proteid  are  most  closely  associated. 
We  have  abundant  authority  to  show  that  diastase  is  associated 
with  leucosin  ;  rennin  is  associated  with  hetero-proteose ;  bromelin 
appears  in  close  relation  to  two  forms  of  proteids,  and  so  on  through 
the  list  a  close  association  of  the  enzyme  with  a  proteid  body  can  be 
shown.  But  it  cannot  be  said  that  the  proteid  is  actually  the 
enzyme.  So  far  as  our  present  knowledge  goes,  an  analysis  of  the 
proteid  must  stand  for  an  analysis  of  the  enzyme. 
From  the  examination  of  the  water-soluble  contents  of  the  latex 
of  the  papaw,  we  may  reach  the  conclusion  that  the  enzyme  is 
associated  with  one  or  more  of  the  soluble  proteids.  An  analysis  of 
these  proteid  bodies  was  therefore  made,  as  follows : 
For  the  purpose  of  analysis,  a  portion  of  the  air-dried  latex  was 
extracted  with  alcohol,  benzine  and  ether,  to  remove  waxes,  resins, 
etc.,  the  residue  consisting  of  the  proteid  matters  and  ash.  This 
preparation  is  marked  I  in  the  accompanying  table. 
■  15  By  the  digestion  of  a  solution  of  this  peptone  with  the  separated  ferment 
or  with  trypsin,  leucin  and  tyrosin  appear  i  indicating  hemipeptone  |. 
16  The  classification  of  the  albumoses  and  peptones  is  the  subj  ect  of  controversy. 
The  classification  here  followed  is  that  in  most  common  use.  Under  another 
view  we  would  have  in  this  substance  a  mixture  of  globulin,  proto  and  deutero 
albumose  with,  possibly,  two  or  more  forms  of  peptone. 
