Am.  Jour.  Pharru 
November,  1901. 
•}    Preparations  of  the  Suprarenal  Glands. 
533 
fully  anaesthetized  with  chloroform,  ether  or  chloretone.  I  have 
used  the  latter  drug  almost  entirely,  as  but  one  dose,  which  is  given 
per  stomach,  is  required.  In  from  fifteen  to  thirty  minutes  the  ani- 
mal is  thoroughly  anaesthetized,  and  will  remain  entirely  insensible 
to  pain  for  any  length  of  time.  Another  decided  advantage  pos- 
sessed by  this  anesthetic  over  chloroform  and  ether  for  laboratory 
work  is  the  fact  that  the  blood-pressure  remains  constant  for  many 
hours.  After  the  animal  is  completely  anaesthetized  he  is  placed 
on  the  operating  table,  and  glass  conulae  of  suitable  size  are  tied? 
as  quickly  as  possible,  into  the  carotid  artery  and  femoral  vein,  the 
vessels  being  clamped  off  previously  with  forceps.  The  conula  in 
the  artery  is  connected  to  an  inelastic  tube,  completely  filled  with  a 
half-saturated  sodium  carbonate  solution  to  prevent  the  blood  from 
clotting  by  means  of  a  short  piece  of  rubber  tubing,  great  care 
Fig.  2. — Blood-pressure  tracing  obtained  in  the  same  manner  as  No.  r. 
Suprarenal  liquid  solution  was,  however,  more  dilute  than  that  employed  in 
being  exercised  to  exclude  all  air.  The  other  end  of  the  inelastic 
tube  terminates  in  a  U-shaped  glass  manometer  tube  which  is  partly 
filled  with  mercury,  which  has  resting  upon  its  free  surface  a  glass 
float  tipped  with  a  glass  writing  pen  or  stylus.  As  soon  as  all  the 
connections  are  made  between  the  artery  and  manometer,  the  clamp 
employed  to  prevent  the  flowing  of  blood  from  the  vessel  is  removed 
and  immediately  the  float  bearing  the  writing  instrument  begins  to 
rise  and  fall  in  unison  with  the  beats  of  the  heart.  The  recording 
drum,  which  has  been  carefully  placed  in  contact  with  the  writing 
instrument,  is  released  at  the  same  moment  and  a  graphic  record  of 
the  blood-pressure  and  heart  beats  is  made  upon  the  traveling  sheet 
of  paper.  A  few  inches  of  record  are  taken  as  a  normal  tracing. 
Then  a  quantity  of  the  solution  of  the  preparation  of  the  supra- 
renal glands,  representing  a  known  quantity  of  the  product,  is  in- 
jected into  the  vein,  through  the  other  glass  cornula,  care  being 
No.  i. 
