438 
Prinos  Verticellatus, 
Am.  Tour.  Pharm. 
Sept.,  1880. 
Residue  A  was  then  treated  with  ether  as  long  as  the  ether  was 
discolored. 
The  ether  was  distilled  ofF,  over  a  water  bath,  and  the  yellowish 
mass  which  remained  was  precipitated  with  water,  which  precipitate 
proved  to  be  resin  of  a  yellowish  color,  odorless,  tasteless  and  pul- 
verizable. 
The  resin,  by  repeatedly  dissolving  in  alcohol  and  precipitating  in 
water,  was  obtained  nearly  white  j  but  still  having  a  slight  yellowish 
cast,  and  was  soluble  in  alcohol,  ether  and  chloroform. 
Residue  B  was  then  treated  with  water,  in  which  it  was  entirely 
soluble. 
A  portion  of  it  was  treated  with  Tr.  sesquichloride  of  iron,  pro- 
ducing a  greenish-black  precipitate  ;  another  portion  gave  with  solution 
of  gelatin  a  flocculent  precipitate  indicating  the  presence  of  tannin. 
Oxide  of  lead  was  then  added  to  the  whole  solution,  evaporated  to 
dryness,  redissolved  in  water  and  the  excess  of  lead  precipitated  by  sul- 
phuretted hydrogen,  and  filtered. 
To  a  small  quantity  of  this  solution  was  added  a  solution  of  potassic 
mercuric  iodide  ;  this,  however,  did  not  give  a  precipitate,  thus  show- 
ing that  the  bark  does  not  contain  an  alkaloid  as  has  been  heretofore 
supposed. 
Another  portion  of  this  solution,  treated  with  Trommer's  test,  gave 
a  red  precipitate,  showing  presence  of  grape  sugar. 
To  remove  this  sugar  considerable  difficulty  was  experienced  j  agita- 
ting it  with  a  mixture  of  two  parts  ether  and  one  of  alcohol  and  allow- 
ing it  to  subside,  decanting  the  clear  liquid,  and  repeating  the  operation 
until  on  evaporating  a  portion  no  residue  remained. 
Upon  evaporating  this  solution  to  dryness,  and  redissolving  in  water, 
it  still  gave  reaction  with  Trommer's  test  showing  grape  sugar. 
Subacctate  of  lead  was  added  to  the  solution  precipitating  the  bitter 
principle,  leaving  the  sugar  in  solution. 
The  precipitate  was  thoroughly  washed  and  diffused  through  water, 
the  lead  precipitated  with  sulphuretted  hydrogen,  filtered,  and  evapo- 
rated to  dryness.  It  was  then  redissolved  in  water,  purified  animal 
charcoal  added  and  again  carefully  evaporated  to  dryness. 
This  was  exhausted  with  boiling  alcohol  and  concentrated. 
On  allowing  some  of  the  bitter  alcoholic  solution  to  evaporate  spon- 
taneously, crystals  of  cane  sugar  were  obtained.^ 
