352 
RESEARCHES ON EMULSINE. 
sion with three times their weight of water, and the whole 
exposed for twelve hours to a temperature between 68° and 
78°. In this interval a yellowish-white coagnlum separates 
on the surface ; the liquid is of a light yellow color; and fur- 
nishes a precipitate with acetic acid, which however no 
longer appeared after it had been kept for two or three days. 
Alcohol furnishes a precipitate which entirely redissolves 
on the addition of water. As soon as the coagulum had 
separated, the liquid was filtered, and also the precipitate 
produced by the acetic acid. The emulsine was precipi- 
tated from the filtered solution with alcohol of 0*848 spec, 
grav., of which it acquired twice its volume. 
The emulsine thus obtained, well washed with alcohol 
and dried in the air, is a transparent, gummy, readily-friable 
substance of a dark or reddish -brown color, without odor 
and without any particular taste. It dissolves only after 
long standing or on trituration with water, leaving an inso- 
luble residue, which contains, besides some phosphate of 
magnesia and some phosphate of lime, a considerable 
amount of organic substance. This insoluble residue, even 
when completely washed with distilled water so as to re- 
move all soluble matter, still furnishes the characteristic re- 
action of emulsine with amygdaline. Four analyses of this 
residue furnished the following results : — 
I. 
II. 
III. 
IV. 
Organic substance 
56 
69-45 
59-48 
SO-27 
Ash 
44 
30-55 
40-52 
10-73 
The solution of this emulsine is opalescent, and very 
readily converts amydgaline to oil of bitter almonds and 
prussic acid. 
Emulsine which has been dried in vacuo over sulphuric 
acid has nearly the appearance above described ; in general 
it is not so dark, sometimes having a brownish yellow tint, 
and in other cases a brownish-red. When dried in the 
air, in proportion as the alcohol escapes it absorbs moisture 
with great avidity. 
The precipitate of emulsine acquires a totally different 
