354 RESEARCHES ON EMULSINE. 
composer], with a disagreeable odor 5 aprecipitate separates, 
but it no longer becomes acid, 
Emulsine does not coagulate, but at a boiling heat, it be- 
haves in a very peculiar manner; between 95° and 96° its 
solution becomes turbid, at 113° it becomes opake and 
milky, and between 185° and 212° it gradually deposits a 
snow-white granular precipitate. If the liquid is boiled for 
a few minutes, the filtered solution, each time that it is 
heated to boiling,becomes quite opake, and deposits a copi- 
ous flocculent precipitate ; on cooling, this entirely redis- 
solves. This experiment can be repeated several times with 
the same result. The granular precipitate first formed 
amounts to about 10 per cent, of the emulsine employed ; 
it is perfectly white, can be easily reduced into a very fine 
powder, and leaves on incineration a neutral ash, which in 
one experiment amounted to 48-74, and in a second to 
59*11 percent., and consisted of phosphate of magnesia with 
some phosphate of lime. The organic substance combined 
with it contained nitrogen, but no sulphur that could be 
detected in it by potash and a salt of lead. 
The liquid filtered from the precipitate contains two pro- 
ducts of decomposition of emulsine, one of which, forming 
about a fourth of the quantity originally employed, is not 
precipitated by alcohol, whilst the other, constituting about 
30 per cent., separates upon the addition of strong alcohol 
in the form of a white granular precipitate. Washed with 
alcohol and ether and dried, this precipitate forms a white ? 
opake, tenacious mass, which is difficult to powder, and 
contains a large, although variable, amount of the above 
salts of phosphoric acid. Several experiments furnished 
from 18 to 35 per cent, of ash. The analyses gave the fol- 
lowing results, according to which the substance differs es- 
sentially from emulsine : — 
Carbon 43-17 40-11 42-48 
Hydrogen 6S5 6-73 7-92 
Nitrogen 8-62 8-34 8-48 
Oxygen and sulphur 41-36 41-83 42.02 
