RESEARCHES ON EMULSINE. 
355 
Acetate of lead separates this substance into two others, 
one of which contains sulphur, the other none. To the 
aqueous solution of the fresh precipitate obtained by alco- 
hol, acetate of lead was added as long as a precipitate result- 
ed ; this was suspended in water, decomposed by sulphu- 
retted hydrogen gas, and the excess of sulphuretted hydro- 
gen expelled at a gentle heat. The filtered liquid had 
a strong acid reaction, which arose partly from phos- 
phoric acid, partly from an organic substance. Upon 
concentration it formed a syrupy mass, which was decom- 
posed by potash, with evolution of ammonia, and with a 
salt of lead it showed the presence of sulphur. 
The liquid filtered from the precipitate obtained by acetate 
of lead, furnished, after removing the lead and the acetic acid, 
on evaporation, a neutral gummy mass, readily soluble in 
water, and which yielded a jelly with a solution of caustic 
potash. It contained nitrogen, but no sulphur. 
Unboiled emulsine is so completely precipitated from its 
solution by neutral acetate of lead, that the filtered liquid no 
longer furnishes oil of bitter almonds with amygdaline, whilst 
the lead precipitate retains this reaction. 
A solution of emulsine begins, in the course of four or five 
days, by exposure to the air at the ordinary temperature, to 
be decomposed with evolution of gas ; it becomes turbid ; 
gives with neutral acetate of lead, but not with acetic acid, 
a copious precipitate, and still retains for some time the re- 
action with amygdaline. 
A solution of emulsine, which has been freed from the 
phosphates by neutralization with lime-water, yields with 
neutral acetate of lead a thick precipitate. This was decom- 
posed with sulphuretted hydrogen. After filtration and re- 
moval of the excess of sulphuretted hydrogen at a gentle 
heat, an acid liquid was obtained, which left on evaporation 
an acid gummy mass containing nitrogen. It was insoluble 
in alcohol and ether ; it formed with baryta and silver in- 
soluble, with magnesia soluble salts. 
The liquid filtered from the precipitate with acetate of lead 
