346  Assay  of  Nux  Vomica.  {^iS&Egr** 
Calculated  amount  o-ioo  gm.,  showing  an  increase  from  this 
hydro-alcoholic  solution  of  4  per  cent. 
The  residues  were  dissolved  in  chloroform  and  again  evaporated. 
Table  VI. — Result. 
One  Hour.  One  Hour.  One  Hour. 
0-1170  0*116  0*119 
Three  Hours.  Three  Hours.  Three  Hours. 
0-1135  O'HI  o-no 
These  residues  were  then  dissolved  in  chloroform  and  allowed 
to  slowly  evaporate  spontaneously  in  a  cold  glass  case  for  48  hours> 
then  heated  at  1700  F.: 
Table  VII.— Result. 
One  Hour.  One  Hour.  One  Hour. 
0*II20  0*III  O'HI 
Three  Hours.  Three  Hours.  Three  Hours. 
0*1120  O'lII  O'lIO 
Average  of  twelve  experiments,  0-1128. 
It  was,  therefore,  shown  that,  while  5  cc.  of  this  solution  gave  by 
direct  evaporation,  not  less  than  0-104.  gm.  by  evaporation  from 
hydro-alcoholic  solution,  that  the  average  of  twelve  experiments  with 
chloroformic  solution  (Nos.  vi  and  vii)  was  0-1128  gm.  Hence, 
by  chloroformic  rotation  the  total  residue  should  range  between  the 
minimum  o-no  gm.  and  the  maximum  0-119  gm.,  the  average  being 
0-1128  gm. 
To  arrive  at  the  facts,  three  experiments,  5  cc.  each,  were  made 
of  the  afore-named  solution  (No.  v).  The  alcoholic  solution  was 
evaporated  to  dryness  in  a  platinum  dish  and  the  residue  dis- 
solved in  small  portions  of  diluted  sulphuric  acid  (1  to  49)  and 
poured  into  the  rotator,  then  it  wras  made  alkaline  with  ammonia 
and  abstracted  by  successive  portions  of  chloroform,  10  cc.  each  : 
Table  VIII. 
First     rotation,  10  cc.  of  chloroform,   0*102 
Second       "  "  "   0*012 
0*114 
First     rotation,  10  cc.  of  chloroform,  0*1075 
Second       "  "  "   0*007 
0-1145 
